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Ultra-LEAF™ Purified anti-human/mouse/rat CD278 (ICOS) Recombinant Antibody

Pricing & Availability
Clone
QA17A10 (See other available formats)
Regulatory Status
RUO
Other Names
Inducible Costimulator, AILIM, CVID1, CRP1
Isotype
Human IgG1, κ
Ave. Rating
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Product Citations
publications
QA17A10_ULEAF_CD278_Antibody_1_051319
Human peripheral blood lymphocytes were stimulated with PHA for 3 days and stained with CD3 Alexa Fluor® 700 and Ultra-LEAF™ Purified ICOS (clone QA17A10, left) or purified recombinant human IgG1, κ (clone QA16A12, right) followed by anti-human IgG-PE.
  • QA17A10_ULEAF_CD278_Antibody_1_051319
    Human peripheral blood lymphocytes were stimulated with PHA for 3 days and stained with CD3 Alexa Fluor® 700 and Ultra-LEAF™ Purified ICOS (clone QA17A10, left) or purified recombinant human IgG1, κ (clone QA16A12, right) followed by anti-human IgG-PE.
  • QA17A10_ULEAF_CD278_Antibody_2_051319
    C57BL/6 mouse splenocytes were stimulated with ConA + IL-2 for three days and stained with Ultra-LEAF™ purified ICOS (clone QA17A10, filled histogram) or purified recombinant human IgG1, κ (clone QA16A12, open histogram) followed by anti-human IgG-PE.
  • QA17A10_ULEAF_CD278_Antibody_3_051319
    Lewis rat splenocytes were stimulated with ConA + IL-2 for three days and stained with Ultra-LEAF™ purified ICOS (clone QA17A10, filled histogram) or purified recombinant human IgG1, κ (clone QA16A12, open histogram) followed by anti-human IgG-PE.
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395103 100 µg 252€
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395104 1 mg 595€
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Description

CD278, also know as ICOS, is a type I transmembrane protein, member of the CD28/CTLA-4 family, present in the cell surface as an homodimer of 47-57 kD. ICOS is expressed in activated T cells where it has a pivotal role in T cell activation, proliferation and Th1/Th2 differentiation and the antibody response; its ligand is CD275.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Recombinant
Host Species
Human
Immunogen
Mouse T cell clone D10.G4.1
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2810589 (BioLegend Cat. No. 395103)
AB_2810590 (BioLegend Cat. No. 395104)

Antigen Details

Structure
Type I transmembrane protein, member of the CD28/CTLA-4 family, homodimer of 47-57 kD.
Distribution

Activated T cells

Function
Pivotal role in T cell activation, proliferation and Th1/Th2 differentiation; required for humoral immune response.
Ligand/Receptor
B7-H2 (ICOS-L, CD275)
Cell Type
T cells, Th17, Thymocytes, Tregs
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References
  1. Tahiliani V, et al. 2017. J Immunol. 198:218.
  2. Pedros C, et al. 2016. Nat Immunol 17:1005.
  3. Maazi H, et al. 2015. Immunity 42:538.
  4. Guedan S, et al. 2014. Blood. 124:1070.
  5. Weber JP, et al. 2015. J. Exp. Med. 212:217.
Gene ID
29851 View all products for this Gene ID
UniProt
View information about CD278 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 05/13/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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