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Deep Blue Cell Viability™ Kit

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Regulatory Status
RUO
Other Names
Resazurin
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Product Citations
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FA_DeepBlueCellViability_Kit_083115
Detection of Baf3/CCR3 cells viability using Resazurin fluorescence measurement. The increasing cell numbers correlate with the increasing fluorescence.
  • FA_DeepBlueCellViability_Kit_083115
    Detection of Baf3/CCR3 cells viability using Resazurin fluorescence measurement. The increasing cell numbers correlate with the increasing fluorescence.
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424701 25 mL 52€
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424702 100 mL 90€
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Description

The Deep Blue Cell Viability™ Kit is formulated to study cell proliferation and quantification. The extent of resazurin reduction and resorufin production is proportional to the number of metabolically active cells (live cells) present in the culture. The Deep Blue Cell Viability™ Kit can facilitate cytotoxicity studies in high-throughput screening, as well as the evaluation of new drugs and chemicals.

Product Details
Technical Data Sheet (pdf)

Product Details

Formulation
Aqueous solution.
Preparation
The solution is filtered through a 0.22 µm membrane that contains no preservatives.
Storage & Handling
Store at 2°C to 8°C for up to 12 months.
Application

FA - Quality tested
Recommended Usage

Add 10% of the Deep Blue Cell Viability™ Kit to the wells containing the cells to study.

For example: in a well containing 100 µl of cell culture, add 10 µl of the Deep Blue Cell Viability™ reagent. Incubate as needed depending on the cell type, which is typically one - six hours.

Application Notes

The Deep Blue Cell Viability™ Kit is based on the resazurin reagent. The typical blue color of resazurin is converted to fluorescence by the action of metabolic enzymes. The reduction of resazurin into resorufin can be detected using a fluorometer (Excitation: 530-570 nm, Emission = 590-620 nm).

Application References

(PubMed link indicates BioLegend citation)
  1. Nakayama GR, et al. 1997. J. Immunol. Methods. 2:205.
  2. Wilson I, et al. 2000. Eur. J. Biochem. 17:5421.
Product Citations
  1. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  2. Taft J, et al. 2021. Cell. 184(17):4447-4463.e20. PubMed
  3. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  4. Kucab JE, et al. 2019. Cell. 177:821. PubMed

Antigen Details

Biology Area
Cell Biology, Cell Proliferation and Viability, Neuroscience
Gene ID
NA
Go To Top Version: 2    Revision Date: 01/08/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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