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Purified anti-GluR1 Phospho (Ser845) Antibody (Previously Covance catalog# PRB-509P)

Pricing & Availability
Clone
Poly28025 (See other available formats)
Regulatory Status
RUO
Other Names
Glutamate receptor 1, GluR1, glutamate receptor A, glutamate receptor subunit GluR1, AMPA-selective glutamate receptor 1, glutamate receptor, ionotropic, AMPA1 (alpha 1), GLUH1, GLUR1, GLURA, GluA1, HBGR1, gluR-1, gluR-A, gluR-K1
Previously
Covance Catalog# PRB-509P
Isotype
Rabbit Polyclonal IgG
Ave. Rating
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Product Citations
publications
Poly28025_Purified_GluR1_Phospho_Ser845_Antibody_IHC_022315
Image showing increased phosphorylation of GluR1 at the S845 site at synapses in the spinal cord in vivo ten minutes after an acute noxious stimulus in an anesthetized rat, stained with Cat No. 802501 & GluR2 (gr). Photo courtesy of A. Todd.
  • Poly28025_Purified_GluR1_Phospho_Ser845_Antibody_IHC_022315
    Image showing increased phosphorylation of GluR1 at the S845 site at synapses in the spinal cord in vivo ten minutes after an acute noxious stimulus in an anesthetized rat, stained with Cat No. 802501 & GluR2 (gr). Photo courtesy of A. Todd.
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802501 150 µL 312€
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Description

GluR1 belongs to the family of Glutamate Receptors (GluRs) that are essential for regulating excitatory synaptic transmission by L-glutamate in the CNS of vertebrates. These are mostly expressed in granule and pyramidal cells in the hippocampus. GluRs can be categorized as ionotropic or metabotropic and subcategorized by their specific agonists (NMDA, AMPA or Kainic acid). There are four types of AMPA related GluR subunits (GluR1, GluR2, GluR3 and GluR4) that are generated by alternative RNA splicing. Tetrameric or pentameric combinations of different subunits contribute to the functional diversity of AMPA receptors.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Rat
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
This antibody was raised against synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho (Ser845) of rat glutamate receptor subunit, GluR1.
Formulation
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, and 50% glycerol.
Preparation
The antibody was purified by affinity chromatography.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

IHC-P, WB, IP, ELISA
Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining.

The optimal working dilution should be determined for each specific assay condition.
• WB: 1:1000 - 1:2500
• IHC-P: 1:500 - 1:1250
• IP: 1:1000 - 1:2500
• ELISA: 1:1000 - 1:2500

Tissue: FFPE Rat Brain
Retrieval: Citrate

Application Notes

These antibodies are effective in immunoblotting (WB), immunohistochemistry (IHC), immunoprecipitation (IP), and ELISA.

*Predicted MW=100 kD

This antibody is specific for Mr 100 kD GluR1 phosphorylated at Ser 845. Immunolabeling of the 100 kD band in a rat hippocampal homogenate is blocked by the phosphopeptide but not by the dephosphopeptide. This antibody was purified by sequential chromatography on protein A and phospho- and dephospho- peptide affinity columns.


This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Nagy GG, Al-Ayyan M, Andrew D, Fukaya M, Watanabe M, Todd AJ. Widespread expression of the AMPA receptor GluR2 subunit at glutamatergic synapses in the rat spinal cord and phosphorylation of GluR1 in response to noxious stimulation revealed with an antigen-unmasking method. J Neurosci 24(25):5766-77, 2004. (IHC, IF)
  2. Roche KW, O'Brien RJ, Mammen AL, Bernhardt J, Huganir RL. Characterization of multiple phosphorylation sites on the AMPA receptor GluR1 subunit. Neuron 16:1179-1188, 1996. (IP)
RRID
AB_2564646 (BioLegend Cat. No. 802501)

Antigen Details

Cell Type
Glutamatergic Neurons
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Synaptic Biology
Molecular Family
Postsynaptic proteins, Phospho-Proteins
Gene ID
50592 View all products for this Gene ID
UniProt
View information about GluR1 Phospho Ser845 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 5    Revision Date: 09/01/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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