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Brilliant Violet 421™ anti-human CD289 (TLR9) Antibody

Pricing & Availability
Clone
S16013D (See other available formats)
Regulatory Status
RUO
Other Names
CD289, TLR9, Toll-like receptor 9
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
S16013D_BV421_CD289_antibody_1_011819
Human peripheral blood mononuclear cells were stained with CD304 APC and CD19 FITC, fixed, permeabilized, and then intracellularly stained with CD289 (TLR9) (clone S16013D) Brilliant Violet 421™ (left) or Rat IgG2a, κ Brilliant Violet 421™ isotype control (right). Dot plots exclude monocytes
  • S16013D_BV421_CD289_antibody_1_011819
    Human peripheral blood mononuclear cells were stained with CD304 APC and CD19 FITC, fixed, permeabilized, and then intracellularly stained with CD289 (TLR9) (clone S16013D) Brilliant Violet 421™ (left) or Rat IgG2a, κ Brilliant Violet 421™ isotype control (right). Dot plots exclude monocytes
  • S16013D_BV421_CD289_antibody_2_011819
    Human peripheral blood mononuclear cells were stained with CD304 APC and CD19 FITC, fixed, permeabilized, and then intracellularly stained with CD289 (TLR9) (clone S16013D) Brilliant Violet 421™ (left) or Rat IgG2a, κ Brilliant Violet 421™ isotype control (right). Dot plots exclude monocytes
  • S16013D_BV421_CD289_antibody_3_020519
    Human peripheral blood was stained with CD304 APC, CD19 FITC, and CD289 (clone S16013D) Brilliant Violet 421™ (left) or Rat IgG2a, κ Brilliant Violet 421™ isotype control (right). Dot plots exclude monocytes and granulocytes.
Compare all formats See Brilliant Violet 421™ spectral data
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394805 25 tests 215 CHF
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394806 100 tests 435 CHF
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Description

TLR9 is a member of the toll-like receptor family that aids in recognition of pathogen associated molecular patterns (PAMPs). TLR9 recognizes unmethylated CpG sequences in DNA (ie. intracellular bacteria and DNA viruses). TLR9 signals through the MyD88 pathway leading to inflammatory cytokine production, especially production of type I interferons by pDCs.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
CD289 (TLR9)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC, FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

S16013D clone can be used for both surface and intracellular detection of TLR9. ICFC compatible with both the intracellular flow cytometric staining and True-Nuclear™ transcription buffer set. Does not work for WB (tested on Daudi cell line).

RRID
AB_2801039 (BioLegend Cat. No. 394805)
AB_2801040 (BioLegend Cat. No. 394806)

Antigen Details

Distribution

TLR9 is expressed by B cells and plasmacytoid dendritic cells (pDC).

Ligand/Receptor
TLR9 recognizes unmethylated CpG sequences in DNA.
Cell Type
B cells, Dendritic cells
Biology Area
Bacterial proteins and Toxins, Immuno-Oncology, Immunology, Innate Immunity
Molecular Family
CD Molecules, Innate Immune Signaling, Toll Like Receptors
Antigen References
  1. Hornung V, et al. 2002. J. Immunol. 168:4531.
  2. Eaton-Bassiri A, et al. 2004. Infect. Immun. 72:7202.
  3. Krieg, A. 2007. J. Clin. Invest. 117:1184.
Gene ID
54106 View all products for this Gene ID
UniProt
View information about CD289 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 1    Revision Date: 01.18.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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