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Biotin anti-mouse PCSK9 Antibody

Pricing & Availability
Clone
Poly5251 (See other available formats)
Regulatory Status
RUO
Other Names
Neural Apoptosis Regulated Convertase 1 (NARC1), proprotein convertase PC9, subtilisin/kexin-like protease PC9, FH3, HCHOLA3, AI415265, AI747682
Isotype
Goat Polyclonal Ig
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Poly5251_Biotin_PCSK9_Antibody_022717
  • Poly5251_Biotin_PCSK9_Antibody_022717
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525104 50 µg 375 CHF
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Description

Proprotein convertase subtilisin/kexin 9 (PCSK9) possesses a signal peptide, a prosegment, a catalytic domain, a hinge region, and a C-terminal Cys-His-rich domain. This protein is primarily synthesized and secreted by hepatocytes. Besides the liver, it is also expressed in the small intestine, kidney, and brain. It is also present in plasma. Upon translocation to the endoplasmic reticulum, the prosegment of PCSK9 is autocatalytically cleaved and secreted as a stable, enzymatically inactive, non-covalent complex. Elevated low density lipoprotein-cholesterol (LDLc) level is a major risk factor for cardiovascular disease and atherosclerosis. PCSK9 is a regulator of LDLc levels through the binding of LDLR, which subsequently leads to the degradation of LDLR. PCSK9 null mice fed with a high fat diet demonstrated a four-fold decrease in cholesterol accumulation, which stresses the important role played by PCSK9 in the development of cardiovascular diseases. PCSK9 also binds to other LDLR family members such as very low density lipoprotein receptor (VLDLR), apolipoprotein E receptor (LRP1/APOER), and apolipoprotein receptor 2 (LRP8/APOER2), which leads to their degradation in intracellular acidic compartments. Like the LDLR, gene expression of PCSK9 is positively regulated by sterol regulatory element-binding protein 2 (SREBP-2), which is a transcription factor that is activated in response to cellular cholesterol depletion.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Polyclonal
Host Species
Goat
Immunogen
Recombinant mouse PCSK9
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

ELISA Detection - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by ELISA. For ELISA detection applications, a concentration of 0.2 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of PCSK9 recombinant protein ranging from 4000 to 62.5 pg/mL is recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance.

Application Notes

ELISA Detection: The biotinylated Poly5251 antibody is useful as a detection antibody for a sandwich ELISA assay, when used in conjunction with purified M114G10 (Cat. No. 532402) antibody as the capture antibody.

 

Note: For testing mouse PCSK9 in serum, plasma, cell culture supernatant, cell lysate, tissue homogenate, and other biological fluids, BioLegend's LEGEND MAX™ Kits (Cat. No. 443207 & 443208) are specially developed and recommended.

RRID
AB_2686956 (BioLegend Cat. No. 525104)

Antigen Details

Structure
Monomer. The molecular mass of a full-length and mature protein is 74.8 kD and 53.7 kD, respectively.
Distribution

Hepatocytes, renal cells, neuroepithelioma, and Schwann cells.

Function
PCSK9 regulates LDLR, VLDLR, APOER, and APOER2 levels, and therefore, LDLc levels. PCSK9 is regulated by SREBP-2.
Interaction
Hepatocytes
Ligand/Receptor
LDLR, VLDLR, APOB, LRP1, and LRP8.
Biology Area
Cell Biology, Neuroinflammation, Neuroscience, Signal Transduction
Molecular Family
Enzymes and Regulators
Antigen References

1. Seidah NG, et al. 2003. Proc. Natl. Acad. Sci. USA 100:928.
2. Naureckiene S, et al. 2003. Arch. Biochem. Biophys. 420:55.
3. Costet P, et al. 2008. Trends Biochem. Sci. 33:426.
4. Grefhorst A, et. al. 2008. J. Lipid Res. 49:1303.
5. Tavori H, et al. 2013. Circulation 127:2403.
6. Denis M, et al. 2012. Circulation 125:894.
7. Tibolla G, et al. 2011. Nutr. Metab. Cardiovasc. Dis. 21:835.

Gene ID
100102 View all products for this Gene ID
UniProt
View information about PCSK9 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 0    Revision Date: 03.30.2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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  • Biotin anti-mouse PCSK9

    Poly5251_Biotin_PCSK9_Antibody_022717
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