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Alexa Fluor® 594 anti-Cytokeratin (pan reactive) Antibody

Pricing & Availability
Clone
C-11 (See other available formats)
Regulatory Status
RUO
Other Names
Cytokeratin, Keratin
Isotype
Mouse IgG1, κ
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Product Citations
publications
C-11_A594_anti-Cytokeratin_PanReactive_Antibody_080619
MCF-7 cells were fixed with 1% paraformaldehyde (PFA) for ten minutes at 37°C, permeabilized with 0.5% TritonX-100 buffer for ten minutes at room temperature, and blocked for 30 minutes at room temperature with 5% fetal bovine serum (FBS). Then the cells were stained with 1.25 µg/mL anti-cytokeratin (pan reactive) (clone C-11) Alexa Fluor® 594 (red). Nuclei were counterstained with DAPI (blue). Images were acquired with a 40X objective.
  • C-11_A594_anti-Cytokeratin_PanReactive_Antibody_080619
    MCF-7 cells were fixed with 1% paraformaldehyde (PFA) for ten minutes at 37°C, permeabilized with 0.5% TritonX-100 buffer for ten minutes at room temperature, and blocked for 30 minutes at room temperature with 5% fetal bovine serum (FBS). Then the cells were stained with 1.25 µg/mL anti-cytokeratin (pan reactive) (clone C-11) Alexa Fluor® 594 (red). Nuclei were counterstained with DAPI (blue). Images were acquired with a 40X objective.
Compare all formats See Alexa Fluor® 594 spectral data See high resolution IF data...
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628606 100 µg 347 CHF
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Description

Cytokeratins are intermediate filament proteins that are widely expressed in many tissues. These proteins are vital components of the cytoskeleton and interact with a number of other proteins. Cytokeratins are usually found in the cytoplasm,although some cytokeratins can be found in the nucleus and the nucleolus.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Keratin enriched fraction from human epidermoid carcinoma.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.1 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

The C-11 monoclonal antibody reacts with a conserved epitope of human cytokeratin 4, 5, 6, 8, 10, 13, and 18. This antibody has been shown to be useful for Western blotting and has also been reported to be useful for immunoprecipitation, immunohistochemistry (paraffin sections), immunocytochemistry, and spatial biology (IBEX)7,8.

Application References

(PubMed link indicates BioLegend citation)
  1. Kovarik J. 1988. Int. J. Cancer Suppl. 3:50.
  2. Bartek J, et al. 1991. J. Pathol. 164:215.
  3. Chernyavsky A I, 2007. J. Biol. Chem. doi:10.1074/jbc.M611365200. PubMed
  4. Comito G, et al. 2012. Cancer Lett. 324:31. PubMed
  5. Vojt-òsek B, et al. 1989. Folia Biol (Praha). 35:373. (reactivity with rat, dog, sheep, pig, cow)
  6. Dekaney CM, et al. 2005. Gastroenterology 129:1567. (ICC)
  7. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  8. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Sun YF, et al. 2021. Nat Commun. 12:4091. PubMed
RRID
AB_2566437 (BioLegend Cat. No. 628606)

Antigen Details

Structure
Intermediate filament protein, contains three coiled-coil domains. Cytokeratins exist as heterotetramers composed of two type I and two type II keratin subunits
Distribution

Cytokeratins are widely expressed proteins usually found in the cytoplasm although some cytokeratins can also be found in the nucleus and the nucleolus

Function
Intermediate filament protein involved with the cytoskeleton.
Interaction
Most cytokeratins interact with a number of other proteins such as 14-3-3, EGF receptor, and many others
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Gene ID
3875 View all products for this Gene ID
UniProt
View information about Cytokeratin pan reactive on UniProt.org

Related FAQs

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Go To Top Version: 4    Revision Date: 12.17.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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