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APC anti-human CD180 (RP105) Antibody

Pricing & Availability
Clone
MHR73-11 (See other available formats)
Regulatory Status
RUO
Other Names
CD180, Radio-protective 105-kD protein, RP105
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
MHR73-11_APC_CD180_Antibody_100218
Human peripheral blood lymphocytes were stained with CD180 (clone MHR73-11) APC (filled histogram) or mouse IgG1, κ isotype control APC (open histogram).
  • MHR73-11_APC_CD180_Antibody_100218
    Human peripheral blood lymphocytes were stained with CD180 (clone MHR73-11) APC (filled histogram) or mouse IgG1, κ isotype control APC (open histogram).
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312911 25 tests £102
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312912 100 tests £195
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Description

CD180, also known as RP105, is a 105 kD member of the toll-like receptor (TLR) family and is expressed on most B cells, monocytes/macrophages and dendritic cells. The TLR family's structural characteristics are critically involved in protein-protein signaling interactions leading to the activation of the innate immune system. Additionally, MD-1, a secretory protein, associates with CD180 (RP105) at the cell surface and this complex, in conjunction with TLR4, recognizes bacterial LPS. The human CD180 (RP105) gene maps to 5q12. The MHR73-11 antibody is able to activate B cells and to protect against irradiation- or dexamethasone-induced apoptosis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
Baboon, African Green
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human RP105 complexed with mouse MD-1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: intracellular staining1, immunoprecipitation1, immunohistochemistry1 of acetone-fixed frozen tissue sections, B cell activation1, protection from apoptosis induced by radiation or dexamethasone, and induction of the costimulatory molecule CD80 (B7-1)1. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 312913 and 312914).

Application References

(PubMed link indicates BioLegend citation)
  1. Miura Y, et al. 1998. Blood 92:2815.
  2. Indrevaer RL, et al. 2013. J. Immunol. 191:3624. PubMed
RRID
AB_2783186 (BioLegend Cat. No. 312911)
AB_2783187 (BioLegend Cat. No. 312912)

Antigen Details

Structure
Member of the toll-like receptor family, 105 kD
Distribution

B cells, monocytes/macrophages, and dendritic cells

Function
Signaling protein involved in innate immunity
Ligand/Receptor
LPS
Cell Type
B cells, Dendritic cells, Macrophages, Monocytes
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Miura Y, et al. 1998. Blood 92:2815.

Gene ID
4064 View all products for this Gene ID
UniProt
View information about CD180 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 10/02/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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