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Ultra-LEAF™ Purified anti-human S100A8/A9 Heterodimer Antibody

Pricing & Availability
Clone
A15105B (See other available formats)
Regulatory Status
RUO
Other Names
Calprotectin, Human S100A8 (Myeloid-related protein, MRP 8), S100A9 (MRP14), calgranulin A and B, L1, cystic fibrosis antigen, and 27E10 antigen, P8, MIF, NIF, CAGA, CFAG, CGLA, L1Ag, MRP8, CP-10, MA387, 60B8AG
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A15105B_ULEAF_S100A8slashA9_Heterodimer_Antibody_052517
Recombinant human S100A8/A9 heterodimer stimulates proliferation of astrocytes in a dose-dependent manner (black circles). Proliferation induced by human S100A8/A9 heterodimer is neutralized (purple circles) by increasing concentrations of anti-human S100A8/A9 monoclonal antibody (Clone A15015B). The ND50 is typically 2 - 8 µg/mL.
  • A15105B_ULEAF_S100A8slashA9_Heterodimer_Antibody_052517
    Recombinant human S100A8/A9 heterodimer stimulates proliferation of astrocytes in a dose-dependent manner (black circles). Proliferation induced by human S100A8/A9 heterodimer is neutralized (purple circles) by increasing concentrations of anti-human S100A8/A9 monoclonal antibody (Clone A15015B). The ND50 is typically 2 - 8 µg/mL.
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697503 100 µg £185
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697504 1 mg £490
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Description

S100A8/A9, also known as Calprotectin, is a heterodimeric protein that belongs to the family of low molecular weight, calcium binding S100 proteins with two EF-hand calcium binding domains. Calcium and zinc binding influences the S100A8/A9 conformation and oligomerization state, which includes the self-assembly into homo- and heterodimers and larger oligomers. S100A8/A9 is elevated in blood and other body fluids in many pathological conditions including rheumatoid arthritis, inflammatory bowel diseases, viral or microbial infections, and tumors. S100A8/A9 is identified as an endogenous activator of TLR4 and is involved in promoting the inflammatory response in infections. Neutrophil-derived S100A8/A9 inhibits growth of Staphylococcus aureus through the chelation of manganese and zinc. S100A8/A9 is also identified as a potent amplifier of inflammation in autoimmunity as well as in cancer development and tumor spread. Injury induced by S100A8/A9 stimulates IP-10 production in monocytes and macrophages. In addition, the S100A8/A9 heterodimer also promotes astrocyte proliferation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human A100A8/A9
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Block - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by blocking activity. The ND50 is 2 - 8 µg/mL.  It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2687183 (BioLegend Cat. No. 697503)
AB_2687184 (BioLegend Cat. No. 697504)

Antigen Details

Structure
Non-covalent heterodimer.
Distribution

Neutrophils, monocytes, macrophages, gingival keratinocytes, granulocytes, osteoclasts, fibroblasts, and microvascular endothelial cells.

Function
Pro-inflammatory molecule that possesses antimicrobial properties. Is up-regulated in neutrophils and monocytes at the sites of inflammation.
Interaction
Astrocytes, neutrophils, and monocytes.
Ligand/Receptor
RAGE, TLR4
Cell Type
Neutrophils, Monocytes, Macrophages, Granulocytes, Osteoclasts, Fibroblasts, Endothelial cells
Biology Area
Cell Biology, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family
Growth Factors, Cytokines/Chemokines
Antigen References

1. Sampson B, et al. 2002. Lancet. 360:1742-5.
2. Vogl T, et al. 2007. Nat. Med. 13:1042-9.
3. Semprini S, et al. 2002. Hum. Genet. 111:310-3.
4. Corbin BD, et al. 2008. Science 319:962-5.
5. Gopal R, et al. 2013. Am. J. Respir. Crit. Care Med. 188:1137-46.
6. Siegenthaler G, et al. 1997. J. Biol. Chem. 14:9371-7.
7. Ryckman C, et al. 2003. J. Immunol. 170:3233-42.
8. Ryu MJ, et al. 2012. J. Biol. Chem. 287:22948-58.
9. Ehrchen JM, et al. 2009. J. Leuko. Biol. 3:557-66.
10. Wang J, et al. 2015. FASEB J. 29(1):250-62.
11. Champaiboon C, et al. 2009. J. Biol. Chem. 284:7078.

Gene ID
6279 View all products for this Gene ID
UniProt
View information about S100A8/A9 Heterodimer on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 0    Revision Date: 05/26/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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