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HRP Goat anti-rat IgG (minimal x-reactivity) Antibody

Pricing & Availability
Clone
Poly4054 (See other available formats)
Regulatory Status
RUO
Isotype
Goat Polyclonal IgG
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405405 500 µL £97
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Description

Goat anti-rat IgG antibody reacts primarily with the heavy chains of rat IgG, but also partially binds to the light chains common to most rat immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rabbit, human, bovine, horse, and mouse immunoglobulins, but it may cross-react with other immunoglobulins from other species.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Rat
Antibody Type
Polyclonal
Host Species
Goat
Formulation
Aqueous buffered solution containing a carrier protein and preservatives.
Preparation
The polyclonal antibody was purified from goat antiserum by rat immunoglobulin affinity chromatography.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

To obtain lot-specific expiration date, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

This product has a shelf-life of 12 months or less. Please use our Expiration Lookup Tool to verify the expiration date of your lot of product
Application

ELISA - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. Do not use this product beyond the expiration date. For ELISA or Western blot analysis, the reagent should be titrated between 1:1,000 - 1:5,000 to determine optimal conditions. For IHC on tissue sections, the reagent should be titrated between 1:500 - 1:2000 to determine optimal conditions. It is recommended that the reagent be titrated for optimal performance for each application. Avoid using diluent solutions containing sodium azide. Sodium azide is an inhibitor of horseradish peroxidase.

Application Notes

This goat anti-rat IgG antibody is useful for direct immunofluorescent staining or as a second step reagent for indirect immunofluorescent staining when used in conjunction with primary antibodies.

Additional Product Notes

This HRP conjugated goat anti-rat IgG antibody is useful as a second step reagent for indirect immunohistochemistry staining when used in conjunction with primary antibodies.

Application References

(PubMed link indicates BioLegend citation)
  1. Zornetzer GA, et al. 2010. J. Virol. 84:11297. PubMed
  2. Frank NY, et al. 2011. Cancer Res. 71:1474. (IHC) PubMed
  3. Baba T, et al. 2020. eLife. 9:e54257. (IHC-P)
Product Citations
  1. Mamedov T, et al. 2019. PLoS One. 14:e0213438. PubMed
  2. Ma Y, et al. 2021. Sci Rep. 11:13471. PubMed
  3. Malik TH, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  4. van Tok MN, et al. 2018. Front Immunol. 9:1550. PubMed
  5. Baba T, et al. 2021. Cell Death Dis. 12:322. PubMed
  6. Domenger A, et al. 2022. EMBO Mol Med. 14:e14740. PubMed
  7. Kim DH, et al. 2018. Nat Commun. 9:503. PubMed
  8. Mamedov T, et al. 2021. Front Plant Sci. 12:742875. PubMed
  9. Lou Q, et al. 2019. J Immunother Cancer. 7:210. PubMed
  10. Sahlberg S, et al. 2014. PLoS One. 9:94621. PubMed
  11. Snelling T, et al. 2022. Biochem J. :. PubMed
  12. Lu SW, et al. 2020. Nat Commun. 3.660416667. PubMed
  13. Link E, et al. 2017. Vaccine. 10.1016/j.vaccine.2017.08.019. PubMed
  14. Callejas BE, et al. 2021. Front Immunol. 12:744738. PubMed
  15. Zuo X, et al. 2014. PLoS One. 9:84748. PubMed
  16. Freidl R, et al. 2016. J Allergy Clin Immunol. S0091-6749(16)31348-3. PubMed
  17. Tuncel J, et al. 2016. PLoS One. 11: 0155936. PubMed
  18. Mamedov T, et al. 2019. Sci Rep. 9:9868. PubMed
  19. Hayashi M, et al. 2021. Viruses. 13:. PubMed
  20. Siddiqui S, et al. 2016. J Biol Chem. 292:1029-1037. PubMed
RRID
AB_315016 (BioLegend Cat. No. 405405)

Related FAQs

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Go To Top Version: 5    Revision Date: 12/12/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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