- Clone
- CTD110.6 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- O-Linked N-Acetylglucosamine
- Isotype
- Mouse IgM, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
838006 | 100 µg | £278 |
β-O-linked N-acetylglucosamine (O-GlcNAc) is an abundant post-translational modification occuring on serine and threonine residues. The modification has been detected on a variety of nuclear and cytoplasmic proteins involved in diverse cellular functions. Studies to elucidate the role of O-GlcNAc-ylation have been hampered by the lack of a sensitive, specific assay to identify or purify proteins bearing O-GlcNAc.
Product DetailsProduct Details
- Verified Reactivity
- All Species
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- The antibody was raised against a synthetic peptide containing Serine-O-GlcNAc.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
IHC-P - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, the suggested use of this reagent is 5.0 - 10 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
This antibody detects Ser-O-GlcNAc and Thr-O-GlcNAc but shows no cross-reactivity with peptide determinants or other closely-related carbohydrate antigens. Interaction between the antibody and the Ser- or Thr-O-GlcNAc epitope can be blocked by competition with free GlcNAc. Milk proteins may cross-react with the antibody therefore we recommend using BSA or goat serum as a blocker.
Additional reported applications (for the relevant formats) include: ELISA2, immunocytochemistry7, immunoprecipitation2,5,6, and Western blotting3,4,7. -
Application References
(PubMed link indicates BioLegend citation) -
- Comer FI, et al. 2001. Anal. Biochem. 293:169.
- Hart GW, et al. 1995. Adv. Exp. Med. Biol. 376:115. (ELISA, WB, IP)
- Anthonisen EH, et al. 2010. J. Biol. Chem. 285:1607. (WB)
- Dias WB, et al. 2009. J. Biol. Chem. 32:21327. (WB)
- Wang Z, et al. 2007. Mol. Cell Proteomics 8:1365. (IP)
- Kim SJ, et al. 2003. J. Biol. Chem. 43:41890. (IP)
- Kneass ZT, Marchase RB. 2004. J. Biol. Chem. 44:45759. (WB, ICC, IF)
- RRID
-
AB_2632636 (BioLegend Cat. No. 838006)
Antigen Details
- Structure
- 1046 amino acids with a molecular weight of 117 kD.
- Distribution
-
Cytosol, mitochondrion, nucleus, plasma membrane, and cytoskeleton.
- Function
- Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in cytoplasmic and nuclear proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc), Glycosylates a large and diverse number of proteins including histone H2B, AKT1, EZH2, PFKL, KMT2E/MLL5, MAPT/TAU and HCFC1, Can regulate their cellular processes via cross-talk between glycosylation and phosphorylation or by affecting proteolytic processing.
- Biology Area
- Cell Biology, Transcription Factors
- Gene ID
- 8473 View all products for this Gene ID
- UniProt
- View information about O-GlcNAc on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All O-GlcNAc Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-O-GlcNAc | CTD110.6 | WB,ICC,IHC-P,IP |
Alexa Fluor® 594 anti-O-GlcNAc | CTD110.6 | IHC-P |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-O-GlcNAc
Immunofluorescence staining of CTD110.6 on methanol fixed He... IHC staining of CTD110.6 on formalin-fixed paraffin-embedded... Paraffin-embedded neonatal mouse lung sectioned and stained ... -
Alexa Fluor® 594 anti-O-GlcNAc
Human paraffin-embedded intestine tissue slices were prepare...
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