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Purified anti-β-Amyloid, 1-40 Antibody (Previously Covance catalog# SIG-39140)

Pricing & Availability
Clone
11A50-B10 (See other available formats)
Regulatory Status
RUO
Other Names
AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, Amyloid beta A4 protein, preA4, protease nexin-II, peptidase nexin-II, beta-amyloid peptide, alzheimer disease amyloid protein, cerebral vascular amyloid peptide, APP, Amyloid Precursor Protein
Previously
Signet Catalog# 9140-02
Signet Catalog# 9140-05
Signet Catalog# 9140-10
Covance Catalog# SIG-39140
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
11A50-B10_PURE_betaAmyloid_1-40_IHCP1_050118
IHC staining of purified anti-β-Amyloid, 1-40 antibody (clone 11A50-B10) on formalin-fixed paraffin-embedded Alzheimer's disease brain tissue. Following antigen retrieval using 70% formic acid for 20 minutes at room temperature, the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 11A50-B10_PURE_betaAmyloid_1-40_IHCP1_050118
    IHC staining of purified anti-β-Amyloid, 1-40 antibody (clone 11A50-B10) on formalin-fixed paraffin-embedded Alzheimer's disease brain tissue. Following antigen retrieval using 70% formic acid for 20 minutes at room temperature, the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 11A50-B10_PURE_betaAmyloid_1-40_IHCP2_050118
    IHC staining of purified anti-β-Amyloid, 1-40 antibody (clone 11A50-B10) on formalin-fixed paraffin-embedded Alzheimer's disease brain tissue. Following antigen retrieval using 70% formic acid for 20 minutes at room temperature, the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Compare all formats
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805409 50 µL £77
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805401 200 µL £362
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805402 500 µL £709
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805403 1 mL £1174
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Description

Amyloid beta (Aβ or Amyloid beta) denotes peptides of 36-43 amino acids in length that are crucially involved in Alzheimer's disease as the main component of the amyloid plaques found in the brains of Alzheimer patients. The peptides result from the amyloid precursor protein (APP), which is cut by certain enzymes to yield Aβ. Aβ molecules can aggregate to form oligomers (known as "seeds") which are believed to be able to induce other Aβ molecules to also take the misfolded oligomeric form, leading to a chain reaction akin to a prion infection. The seeds or the resulting amyloid plaques are toxic to nerve cells. The other protein implicated in Alzheimer's disease, tau protein, also forms such prion-like misfolded oligomers, and there is some evidence that misfolded Aβ can induce tau to misfold.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution (no preservatives or carrier proteins).
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Do not store antibody diluted below 50 µg/mL in the absence of protein (i.e.: add 2% bovine serum albumin). The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

IHC-P - Quality tested
DOT Blot, ELISA - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone 11A50-B10 is reactive to the C-terminus of beta amyloid and is specific for the isoform ending at the 40th amino acid.

Application References

(PubMed link indicates BioLegend citation)
  1. Watts JC, et al. 2014. Proc. Natl. Sci. USA 28:10323. (IHC-P) PubMed
  2. Diomede L, et al. 2014. Neurobiol. Dis. 62:521. (Dot Plot) PubMed
  3. Watanabe H, et al. 2012. J Neurosci. 15:5085. (ELISA) PubMed
  4. Portelius E, et al. 2006. J. Proteome Res. 4:1010. (IP)
  5. Vingtdeux V, et al. 2011. FASEB J. 25:219. (ELISA) PubMed
  6. Ohmi K, et al. 2011. PLoS One 11:e27461. (IHC-Other) PubMed
Product Citations
  1. Vingtdeux V, et al. 2011. FASEB J. 25:219-231. PubMed
  2. Watts J, et al. 2014. Proc Natl Acad Sci U S A. 111:10323-10328. PubMed
  3. Lisa Dolfe et al. 2018. Journal of Alzheimer's disease reports. 2(1):27-39 . PubMed
  4. Diomede L, et al. 2014. Neurobiol Dis. 62:521-532. PubMed
  5. Watanabe H, et al. 2012. J Neurosci. 32:5085-5096. PubMed
  6. Voth S, et al. 2020. FASEB J. 9156:34. PubMed
  7. Shi H, et al. 2020. Acta Neuropathol. 139:813. PubMed
  8. Bourassa P, et al. 2020. Neuropathol Appl Neurobiol. 46:458. PubMed
  9. Lee CYD et al. 2018. Neuron. 97(5):1032-1048 . PubMed
  10. Plowey ED, et al. 2022. Acta Neuropathol. 144:143. PubMed
  11. Kim K, et al. 2020. Nat Commun. 0.540972222. PubMed
  12. Shi H, et al. 2020. Acta Neuropathol Commun. 0.473611111. PubMed
  13. Ohmi K, et al. 2011. PLoS One. 6:e27461. PubMed
  14. Parker H, et al. 2020. EMBO Mol Med. 12:e11185. PubMed
  15. Ruiz–Riquelme A, et al. 2018. Acta Neuropathol Commun. 6:26. PubMed
  16. Carreras I, et al. 2019. Sci Rep. 9:10972. PubMed
RRID
AB_2734555 (BioLegend Cat. No. 805409)
AB_2564680 (BioLegend Cat. No. 805401)
AB_2564681 (BioLegend Cat. No. 805402)
AB_2564679 (BioLegend Cat. No. 805403)

Antigen Details

Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
APP/β-Amyloid
Gene ID
351 View all products for this Gene ID
UniProt
View information about beta-Amyloid 1-40 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All β-Amyloid, 1-40 Reagents Request Custom Conjugation
Description Clone Applications
Biotin anti-β-Amyloid, 1-40 11A50-B10 ELISA,WB,IHC,IP
HRP anti-β-Amyloid, 1-40 11A50-B10 IHC-P,Dot Blot,ELISA,IP
Purified anti-β-Amyloid, 1-40 11A50-B10 IHC-P,Dot Blot,ELISA
Go To Top Version: 5    Revision Date: 05/17/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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