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Purified anti-CD230 (Prion) Antibody (Previously Covance catalog# SIG-39805)

Pricing & Availability
Clone
7D9 (See other available formats)
Regulatory Status
RUO
Other Names
Major prion protein, prP27-30, prP33-35C
Previously
Signet Catalog# 9805-02
Signet Catalog# 9805-05
Signet Catalog# 9805-10
Covance Catalog# SIG-39805
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
7D9_PURE_CD230_Antibody_WB_061218
Western blot of purified anti-CD230 (Prion) antibody (clone 7D9). Lane 1: Molecular weight marker; Lane 2: 30 µg of human brain lysate; Lane 3: 30 µg of mouse brain lysate; Lane 4: 30µg of rat brain lysate. The blot was incubated with 1 µg/ml of the primary antibody overnight at 4°C, followed by incubation with HRP labeled Goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 7D9_PURE_CD230_Antibody_WB_061218
    Western blot of purified anti-CD230 (Prion) antibody (clone 7D9). Lane 1: Molecular weight marker; Lane 2: 30 µg of human brain lysate; Lane 3: 30 µg of mouse brain lysate; Lane 4: 30µg of rat brain lysate. The blot was incubated with 1 µg/ml of the primary antibody overnight at 4°C, followed by incubation with HRP labeled Goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
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807904 25 µL £77
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807901 200 µL £398
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Description

Prions cause neurodegenerative disease by aggregating extracellularly within the central nervous system which disrupt the normal tissue structure. This disruption is characterized by "holes" in the tissue with resultant spongy architecture. Two conformational isoforms exist, the normal cellular isoform (PrPC) and the infectious, scrapie isoform (PrPSC). Other histological changes include astrogliosis and the absence of an inflammatory reaction. Neurodegenerative symptoms can include convulsions, dementia, ataxia (balance and coordination dysfunction), and behavioral or personality changes.

All known prion diseases are collectively called transmissible spongiform encephalopathies (TSEs). Prion (PrP) is highly conserved through mammals and comparison between primates ranges from 92.9-99.6% similarity in amino acid sequence. The human protein structure consists of a globular domain with three α-helices and a two-strand antiparallel β-sheet, an NH2-terminal tail, and a short COOH-terminal tail. A glycosylphosphatidylinositol (GPI) membrane anchor at the COOH-terminal tethers PrP to cell membranes. This anchor is integral to the transmission of conformational change; secreted PrP lacking the anchor component is unaffected by the infectious isoform. PrPSC accumulates in compact, protease-resistant aggregates within neural tissue and has a different secondary and tertiary structure from PrPC, but an identical primary sequence.

The primary sequence of PrP is 253 amino acids long before posttranslational modification. Signal sequences in the amino- and carboxy- terminal ends are removed posttranslationally, resulting in a mature length of 208. For human and Syrian hamster PrP, two glycosylated sites exist on helices 2 and 3 at Asn181 and Asn197. Murine PrP has glycosylation sites as Asn180 and Asn196. A disulfide bond exists between Cys179 of the second helix and Cys214 of the third helix (human PrPC numbering).

The precise function of PrP is not yet known, but it is possibly involved in the transport of ionic copper to cells from the surrounding environment. Researchers have also proposed roles for PrP in cell signaling or in the formation of synapses.

Spatial learning, a predominantly hippocampal-function, is decreased in PrP null mice and can be recovered with the reinstatement of PrP in neurons; indicating that loss of PrP function is the cause. PrP is present in both pre- and post-synaptic neuron cells, and the greatest concentration is in the pre-synaptic cells. Some research indicates PrP involvement in neuronal development, differentiation, and neurite outgrowth. The PrP-activated signal transduction pathway is associated with axon and dendritic outgrowth with a series of kinases.

Though most attention is focused on PrP’s presence in the nervous system, it is also abundant in immune system tissue. PrP immune cells include haematopoietic stem cells, mature lymphoid and myeloid compartments, and certain lymphocytes; also, it has been detected in natural killer cells, platelets, and monocytes. T cell activation is accompanied by a strong up-regulation of PrP, though it is not requisite. The lack of immuno-response to transmissible spongiform encephalopathies (TSE), neurodegenerative diseases caused by prions, could stem from the tolerance for PrPSc.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was developed using a recombinant mouse PrP protein, aa 23-237.
Formulation
Phosphate-buffered solution (no preservatives or carrier proteins).
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB).

This antibody reacts exceptionally well with bovine, sheep, and mouse PrP. It is 10 fold less reactive to feline, hamster, and human PrP. The antibody recognizes both protease sensitive and protease resistant forms of PrP (after denaturing).

RRID
AB_2715843 (BioLegend Cat. No. 807904)
AB_2564733 (BioLegend Cat. No. 807901)

Antigen Details

Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
Prion (CD230)
Antigen References
  1. Lee DC, et al. 2000. J. Virol. Methods. 84(1):77-89.
  2. Soto C, et al. 2000. Lancet 355 (9199):192-7.
  3. Rubenstein R, et al. 1999. J. Neurovirol. 5(4):401-13.
  4. Aucouturier P, et al. 1999. Neurosci. Lett. 274(1):33-6.
  5. Safar J, et al. 1998. Nat. Med. 4(10):1157-65.
  6. Kascsak RJ, et al. 1997. Immunol. Invest. 26(1-2):259-68.
  7. Kitamoto T, et al. 1991. J. Virol. 65:6292-5.
  8. Kitamoto T, et al. 1987. Lab. Invest. 57:230-6.
  9. Kascsak et al. 1987. J.Virol. 61:3688-93.
Gene ID
19122 View all products for this Gene ID
UniProt
View information about CD230 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 03/12/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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