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Purified anti-human CD107a (LAMP-1) (Maxpar® Ready) Antibody

Pricing & Availability
Clone
H4A3 (See other available formats)
Regulatory Status
RUO
Workshop
P PR-63; BP 473; P P008
Other Names
Lysosome-Associated Membrane Protein 1, LGP-120, LAMP-1
Isotype
Mouse IgG1, κ
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Product Citations
publications
H4A3_Purified_CD107a_Cytof_1_060314.jpg
Human PBMCs were incubated for 5 hours in media alone (top) or with PMA and Ionomycin (bottom) in the presence of monensin, brefeldin A and 151Eu-anti-CD107a (H4A3). Cells were then fixed, permeabilized, and stained with 165Ho-anti-IFNγ(B27). CD8+ T cells are displayed in the analysis. Data provided by DVS Sciences.
  • H4A3_Purified_CD107a_Cytof_1_060314.jpg
    Human PBMCs were incubated for 5 hours in media alone (top) or with PMA and Ionomycin (bottom) in the presence of monensin, brefeldin A and 151Eu-anti-CD107a (H4A3). Cells were then fixed, permeabilized, and stained with 165Ho-anti-IFNγ(B27). CD8+ T cells are displayed in the analysis. Data provided by DVS Sciences.
  • H4A3_Purified_CD107a_Cytof_2_060314.jpg
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328635 100 µg £97
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Description

CD107a, also known as Lysosome-Associated Membrane Protein 1 (LAMP-1) or LGP-120, is a 110-140 kD type I membrane glycoprotein. Mature CD107a is heavily glycosylated from a 40 kD core protein. This molecule is located on the luminal side of lysosomes. Upon activation, CD107a is transferred to the cell membrane surface of activated platelets, activated lymphocytes, macrophages, epithelial cells, endothelial cells, and some tumor cells. CD107a has been suggested to play a role in the protection of lysosomal membrane from lysosomal hydrolases which is involved in cell adhesion and regulation of tumor metastasis, and mediates autoimmune disease progression. CD107a is a ligand for galaptin and E-selectin. Surface expression of LAMP-1 has been shown to correlate with CD8+ T cell and NK cell cytotoxicity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human adult adherent peripheral blood cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
CyTOF® - Verified
Recommended Usage

This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.

Application Notes

Additional reported applications (for the relevant formats) include: Western blotting8, immunohistochemical staining2, immunofluorescence5,7, and immunoprecipitation5.

This antibody is specific to human LAMP-1. Positive control: Hela cells; LAMP-1 molecular weight appears to be at ~110 kDa on the gel due to high glycosylation.

Additional Product Notes

Maxpar® is a registered trademark of Standard BioTools Inc.

Application References

(PubMed link indicates BioLegend citation)
  1. Misse D, et al. 1999. Blood 93:2454.
  2. Furuta K, et al. 2001. Am. J. Pathol. 159:449. (IHC)
  3. Watanabe A, et al. 2011. J. Biol. Chem. 286:10702. PubMed
  4. Baron Gaillard CL, et al. 2011. Mol. Cell. Biol. 22:5459. PubMed
  5. Hauck CR and Meyer TF. 1997. FEBS Lett. 405:86. (IF, IP)
  6. De Keersmaecker B, et al. 2012. J. Virol. 86:9351. PubMed
  7. Knodler LA, et al. 2010. P. Natl. Acad. Sci. USA. 107:17733. (IF)
  8. Oh J, et al. 2000. Hum. Mol. Genet. 9:375. (WB)
  9. Salio M, et al. 2013 PNAS. 110:4753. PubMed
RRID
AB_2563708 (BioLegend Cat. No. 328635)

Antigen Details

Structure
LAMP-1 is a 417 amino acid protein with a molecular mass of 45 kD.
Distribution

Macrophages, epithelial cells, endothelial cells, some tumor cells; located on the luminal side of lysosomes or on the surface of cell membranes

Function
Protect lysosomal membrane from lysosomal hydrolases, adhesion
Ligand/Receptor
Galaptin
Cell Type
Endothelial cells, Epithelial cells, Macrophages
Biology Area
Cell Biology, Immunology, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Sarafian V, et al. 2006. Arch. Dermatol. Res. 298:7381.
2. Schlossman SF, et al. 1995. Leukocyte Typing V:White Cell Differentiation Antigens. New York:Oxford University Press.
3. Sawada R, et al. 1993. J. Biol. Chem. 268:12675.
4. Chen JW, et al. 1988. J. Biol. Chem. 263:8754.
5. Chen JW, et al. 1986. Biochem. Soc. Symp. 51:97112.

Gene ID
3916 View all products for this Gene ID
UniProt
View information about CD107a on UniProt.org

Related FAQs

Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?

We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.

http://techsupport.fluidigm.com/

Can I use Maxpar® Ready format clones for flow cytometry staining?

We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.

I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.

We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/

Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?

The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.

Go To Top Version: 2    Revision Date: 07/22/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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