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Purified anti-FAK Antibody

Pricing & Availability
Clone
W16060A (See other available formats)
Regulatory Status
RUO
Other Names
Focal adhesion kinase 1, FAK1, FRNK, Protein-tyrosine kinase 2, PTK2
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W16060A_PURE_FAK_Antibody_1_WB_011217
Total cell lysates (15 µg protein) from RPMI8826 (negative control cell line), HeLa, PC3 and NIH3T3 were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with 1 µg/mL purified anti-FAK (clone W16060A) antibody. Proteins were visualized using a goat anti-rat-IgG secondary antibody conjugated to HRP (clone Poly4054) and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin (clone 2F1-1) antibody was used as a loading control.
  • W16060A_PURE_FAK_Antibody_1_WB_011217
    Total cell lysates (15 µg protein) from RPMI8826 (negative control cell line), HeLa, PC3 and NIH3T3 were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with 1 µg/mL purified anti-FAK (clone W16060A) antibody. Proteins were visualized using a goat anti-rat-IgG secondary antibody conjugated to HRP (clone Poly4054) and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin (clone 2F1-1) antibody was used as a loading control.
  • W16060A_PURE_FAK_Antibody_2_ICC_031819
    HeLa cells were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for three minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with 0.5 µg/ml anti-FAK antibody (clone W16060A) overnight at 4°C followed by Alexa Fluor® 594 (red) conjugated goat anti-rat IgG (clone Poly4054) for one hour at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
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694001 25 µg £70
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694002 100 µg £182
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Description

FAK (Focal adhesion kinase) is an intracellular, highly conserved, nonreceptor tyrosine kinase encoded by PTK2 located on human chromosome 8q24.3. It is ubiquitously expressed in all cells and was initially identified in v-Src transformed chicken embryo fibroblasts. FAK is a multi-functional regulator of cell signaling within the tumor microenvironment. During development and in various tumors, FAK promotes cell motility, survival and proliferation through kinase-dependent and -independent mechanisms. FAK is associated with many aspects of metastasis such as adhesion, migration and invasion. FAK is overexpressed and activated in a variety of cancers including ovary, colon, breast, lung, thyroid, head and neck, liver, pancreatic and esophageal and is correlated with poor survival rates. Small molecule FAK inhibitors are emerging as promising chemotherapeutics, as FAK inhibition in mouse models prevents tumor growth, metastasis, vascular permeability, and angiogenesis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial human FAK (701-950 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per ml. For iimmunocytochemistry, a concentration range of 0.5 - 2.0 μg per ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone W16060A does not cross-react with mouse (in-house tested).

Product Citations
  1. Jordan KR, et al. 2020. Breast Cancer Res. 1.005555556. PubMed
RRID
AB_2650721 (BioLegend Cat. No. 694001)
AB_2650722 (BioLegend Cat. No. 694002)

Antigen Details

Structure
1052 amino acids with a predicted molecular weight of 119 kD.
Distribution

Cell junction, cell membrane, cytoplasm.

Function
Plays an essential role in regulating cell migration, adhesion, spreading, reorganization of the actin cytoskeleton, formation and disassembly of focal adhesions and cell protrusions, cell cycle progression, cell proliferation and apoptosis.
Interaction
Interacts with CAS family members, including BCAR1, BCAR3, CASS4 and NEDD9. Interacts with GIT1, SORBS1, ARHGEF28, SHB, PXN, TLN1, STAT1, DCC, WASL, ARHGEF7, GRB2 and GRB7, BMX, TGFB1I1, STEAP4, ZFYVE21, ESR1, PIK3R1 or PIK3R2, SRC, FGR, FLT4, RET, EPHA2.
Biology Area
Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Signal Transduction
Molecular Family
Protein Kinases/Phosphatase
Antigen References

1. Kanteti R, et al. 2016. Oncotarget. 7:31586.
2. Schaller MD, et al. 1992. Proc. Natl. Acad. Sci. USA 89:5192.
3. Sulzmaier FJ, et al. 2014. Nat. Rev. Cancer 14:598.
4. Parsons JT. 2003. J. Cell Sci. 116:1409.
5. Zhao J, Guan JL. 2009. Cancer Metastasis Rev. 28:35.
6. Goode EL, et al. 2010. Nat. Genet. 42:874.
7. Cancer Genome Atlas Network. 2012. Nature 490:61.
8. Sood AK, et al. 2010. J. Clin. Invest.  120:1515.
9. Ward KK, et al. 2013. Clin. Exp. Metastasis 30:579.
10. Jean C, et al. 2014. J. Cell Biol. 204:247.
11. Cabrita MA, et al. 2011. Mol. Oncol. 5:517.

Gene ID
5747 View all products for this Gene ID
UniProt
View information about FAK on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 01/16/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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