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Purified anti-APP C-Terminal Fragment Antibody (Previously Covance catalog# SIG-39152)

Pricing & Availability
Clone
C1/6.1 (See other available formats)
Regulatory Status
RUO
Other Names
AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, Amyloid beta A4 protein, preA4, protease nexin-II, peptidase nexin-II, beta-amyloid peptide, alzheimer disease amyloid protein, cerebral vascular amyloid peptide, APP, Amyloid Precursor Protein
Previously
Covance Catalog# SIG-39152
Isotype
Mouse IgG1
Ave. Rating
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Product Citations
publications
C1slash6pt1_PURE_APP_CTermFrag_WB_052418
Western blot of purified anti-APP C-Terminal Fragment antibody (clone C1/6.1). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • C1slash6pt1_PURE_APP_CTermFrag_WB_052418
    Western blot of purified anti-APP C-Terminal Fragment antibody (clone C1/6.1). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
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802803 25 µL $112
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802801 200 µL $546
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802802 1 mL $1805
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Description

APP functions as a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. It is involved in cell mobility and transcription regulation through protein-protein interactions and cleaved either by alpha-secretase, beta-secretase or theta-secretase, leading to generation and extracellular release of soluble APP peptides, S-APP-alpha and S-APP-beta, and the retention of corresponding membrane-anchored C-terminal fragments, C80, C83 and C99. Subsequent processing of C80 and C83 by gamma-secretase yields P3 peptides. This is the major secretory pathway and is non-amyloidogenic. Alternatively, presenilin/nicastrin-mediated gamma-secretase processing of C99 releases the amyloid beta proteins, amyloid-beta 40 and amyloid-beta 42, major components of amyloid plaques, and the cytotoxic C-terminal fragments, gamma-CTF(50), gamma-CTF(57) and gamma-CTF(59). Many other minor beta-amyloid peptides, beta-amyloid 1-X peptides, are found in cerebral spinal fluid (CSF) including the beta-amyloid X-15 peptides, produced from the cleavage by alpha-secretase and all terminating at Gln-686.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was raised against the conserved carboxyl-terminal 20 residues of APP (residues 676-695 of APP695).
Formulation
Phosphate-buffered solution (no preservatives or carrier proteins).
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB - Quality tested
ICC, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB), immunocytochemistry (ICC), and immunoprecipitation (IP).

Application References

(PubMed link indicates BioLegend citation)
  1. Lee MH, et al. 2010. Cell Death Dis. e110.
  2. Jiang Y, et al. 2010. Proc. Natl. Acad. Sci. USA. 107(4):1630-5. (WB, ICC) PubMed
  3. Laudon H, et al. 2004. J. Biol. Chem. 279(23):23925-32. (IP)
  4. Grbovic OM, et al. 2003. J. Biol. Chem. 278(33):31261-8. (IP)
  5. Mathews PM, et al. 2002. J. Biol. Chem. 277(39):36415-24. (WB)
Product Citations
  1. de Coninck D, et al. 2018. Biophys J. 114:1128. PubMed
  2. Sogorb-Esteve A, et al. 2018. Mol Neurobiol. 55:5047. PubMed
  3. Arber C, et al. 2019. Brain Commun. 1:fcz024. PubMed
  4. Chae CW, et al. 2020. Br J Pharmacol. 177:3828. PubMed
  5. Miranda AM, et al. 2018. Nat Commun. 9:291. PubMed
  6. Lingel A, et al. 2020. J Biol Chem. 295:17114. PubMed
  7. Lingel A, et al. 2020. J Biol Chem. 295:17114. PubMed
  8. Reichenbach N, et al. 2018. J Exp Med. 215:1649. PubMed
  9. García-Ayllón MS, et al. 2017. Sci Rep. 7:2477. PubMed
  10. Jiwaji Z, et al. 2022. Nat Commun. 13:135. PubMed
  11. Lundgren JL, et al. 2020. BMC Neurosci. 21:06. PubMed
  12. Reichenbach N, et al. 2019. EMBO Mol Med. 11:. PubMed
  13. Koh HS, et al. 2021. Int J Mol Sci. 22:. PubMed
  14. Kotfis K, et al. 2020. British Journal of Pharmacology. 49(1):66-72. PubMed
  15. Liu YC, et al. 2020. Mol Ther. . PubMed
  16. Paschkowsky S, et al. 2016. J Biol Chem. 291: 21903 - 21912.. PubMed
  17. Jiang Y, et al. 2010. Proc Natl Acad Sci U S A. 107:1630-1635. PubMed
  18. Arber C, et al. 2021. Cell Reports. 34(2):108615. PubMed
  19. Jean-Louis T, et al. 2018. Neurobiol Aging. 62:130. PubMed
  20. Bourdenx M, et al. 2021. Cell. 184(10):2696-2714.e25. PubMed
  21. Hung COY et al. 2018. Cell reports. 25(13):3647-3660 . PubMed
  22. Zhuravleva V, et al. 2021. Cell Death Dis. 12:1137. PubMed
  23. Corbett G, et al. 2015. Proc Natl Acad Sci U S A. 112: 8445 - 8450. PubMed
  24. Arber C, et al. 2020. Mol Psychiatry. 25:2919. PubMed
  25. Hung C, et al. 2021. Cell Reports. 35(11):109259. PubMed
  26. Williamson RL, et al. 2017. J Biol Chem. 292:19873. PubMed
RRID
AB_2715853 (BioLegend Cat. No. 802803)
AB_2564648 (BioLegend Cat. No. 802801)
AB_2564647 (BioLegend Cat. No. 802802)

Antigen Details

Structure
Expected MW: APP= 100 kD; alphaCTF & betaCTF= 10 kD
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
APP/β-Amyloid
Gene ID
351 View all products for this Gene ID
UniProt
View information about APP C-Terminal Fragment on UniProt.org
Go To Top Version: 3    Revision Date: 05/24/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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