Science can be tough for both novices and grizzled lab bench veterans. Our monthly video series with Dr. Ken Lau will take you through helpful tips and advice regarding multicolor flow cytometry panel construction and other assays. Stay current on all things science with us.

 

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Episode 10 - Autofluorescence

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Description: This month’s Ken Lau Show takes a look at the issue of autofluorescence, the molecules that create it, and how you can avoid it in your samples.

Links

Autofluorescence blog: https://www.biolegend.com/newsdetail/1182/
Autofluorescence in microscopy: 
https://www.microscopyu.com/references/pdfs/Viegas_etal_Eur_J_Histochem-51-59-2007.pdf
https://www2.cbm.uam.es/confocal/Manuales/autofluorescence.pdf


 

Episode 9 - Zombie Fixable Viability Dyes

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Description: With Halloween fast approaching, we look at our Zombie Fixable Viability Dyes to offer tips for its use in flow cytometry and microscopy. 

Links

Zombie Fixable Viability Dyes: biolegend.com/live_dead
Download the Zombie Dyes App: biolegend.com/iphonesupport
Panel Builder: biolegend.com/panelselector 
Tech support: biolegend.com/contact_technical_service


 

Episode 8 - Fluor Choice and Marker Expression

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Description: On this Ken Lau Show, we look at some more tips for panel building for flow cytometry, like marker expression and bivariate analysis. Keywords: flow cytometry, multicolor, panel building, bivariate, acute myeloid leukemia, expression, BioLegend, Ken Lau, science, tips, help. 

Links:

PE/Dazzle™ 594 promotion and information: biolegend.com/PE-Dazzle594
Flow cytometry for acute myeloid leukemia: https://www.cytometry.org/public/educational_presentations/Cherian.pdf
Expression of common surface molecules on blood cells: Expression_Common_Proteins_121511.pdf
Panel Builder: biolegend.com/panelselector
Tech support: biolegend.com/contact_technical_service


 

Episode 7 - The History of Flow Cytometry and Fluorophores

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Description: You perform flow cytometry all the time, but do you know how this technology came about? This month’s Ken Lau show takes a look at the key milestones of development for both the flow cytometer and fluorophores.

Links:

History of Flow Cytometry: biolegend.com/historyofflow
Legends of Biology: biolegend.com/legends_of_biology


 

Episode 5 - Nuclear Antigen Targets

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Description: On this month's Ken Lau Show, we take a look at some considerations for panel building in nuclear antigen staining, particularly fluorophore size, and methanol/ethanol fixations.

Links:

Flow Cytometry Buffers : https://www.biolegend.com/flow_buffers
PE/Dazzle™ 594 Conjugates : https://www.biolegend.com/PE-Dazzle594
The Tandem : https://www.biolegend.com/the_tandem


 

Episode 4 - Tandem Fluorophores

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Description: There are a lot of misconceptions when it comes to the use of tandem fluors in flow cytometry. This month's episode of the Ken Lau show covers the basics of how tandem works, tips for their use, and our brand new tandem, PE/Dazzle™ 594.

Links:

FluoroFix™ Buffer : https://www.biolegend.com/products/fluorofix-buffer-4934
PE/Dazzle™ 594 Conjugates : https://www.biolegend.com/PE-Dazzle594
The Tandem : https://www.biolegend.com/the_tandem
Tandem Dyes : https://www.biolegend.com/tandem_dyes


 

Episode 3 - FMOs, Autofluorescence, and Isotype Controls

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Description: When performing flow cytometry, you have to have the proper controls in order to understand what's real in your results. This month's episode of the Ken Lau show looks at the importance of autofluorescence, isotype controls, and fluorescence minus one controls. 

Links:

TruStain FcX™ Products : www.biolegend.com/search-results?Keywords=TruStain&k1=TruStain


 

Episode 2 - Panel Building and Tiers

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Description: This month, we cover the fundamental building blocks useful for creating a panel. We discuss the proper controls that should be included with every experiment and how you can break your panel into easily digestible tiers for proper fluor assignment.

Links:

Fluorophore Brightness Chart : https://www.biolegend.com/media_assets/support_resource/Fluorophore_Brightness_Index_120913.pdf
Rainbow Beads Blog: https://www.biolegend.com/newsdetail/1362/
Multicolor Staining Guide: https://www.biolegend.com/multicolor_staining
Panel Building Reference: https://www.ncbi.nlm.nih.gov/pubmed/?term=%3A+Mahnke%2C+Y.D.+and+Roederer%2C+M.+2007.+Clin.+Lab.+Med.+27%3A469
Panel Builder: https://www.biolegend.com/panelselector
Tech Support: https://www.biolegend.com/contact_technical_service


 

Episode 1 - Fluorescent Proteins

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Description: Fluorescent proteins produce fantastic colors and serve as excellent reporters for genes of interest. In our inaugural episode, Dr. Ken Lau takes you through the origin of the first fluorescent protein and how these proteins can affect your panel’s other markers.

Links:

GFP Blog: https://www.biolegend.com/newsdetail/1120/
Spectra Analyzer: https://www.biolegend.com/spectraanalyzer
Multicolor Panel Selector: https://www.biolegend.com/panelselector
Custom Panel Construction: https://www.biolegend.com/custom_panel_construction 


 

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