Science can be tough for both novices and grizzled lab bench veterans. Our monthly video series with Dr. Ken Lau will take you through helpful tips and advice regarding multicolor flow cytometry panel construction and other assays. Stay current on all things science with us.

 

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Episode 10 - Autofluorescence

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Description: This month’s Ken Lau Show takes a look at the issue of autofluorescence, the molecules that create it, and how you can avoid it in your samples.

Links

Autofluorescence blog

Autofluorescence in microscopy: 
https://www.microscopyu.com/references/pdfs/Viegas_etal_Eur_J_Histochem-51-59-2007.pdf
https://www2.cbm.uam.es/confocal/Manuales/autofluorescence.pdf


 

Episode 8 - Fluor Choice and Marker Expression

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Description: On this Ken Lau Show, we look at some more tips for panel building for flow cytometry, like marker expression and bivariate analysis. Keywords: flow cytometry, multicolor, panel building, bivariate, acute myeloid leukemia, expression, BioLegend, Ken Lau, science, tips, help. 

Links:

PE/Dazzle™ 594 promotion and informatiom
Flow cytometry for acute myeloid leukemia
Expression of common surface molecules on blood cells
Panel Builder
Tech support


 

Episode 7 - The History of Flow Cytometry and Fluorophores

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Description: You perform flow cytometry all the time, but do you know how this technology came about? This month’s Ken Lau show takes a look at the key milestones of development for both the flow cytometer and fluorophores.

Links:

History of Flow Cytometry


 

Episode 6 - LEGENDplex™

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Description: This month, Ken Lau hosts his very own game show! He quizzes contestants on the merits and features of BioLegend's brand new product, LEGENDplex™, which is a bead-based multi-analyte flow assay kit.

Links:

LEGENDplex™


 

Episode 3 - FMOs, Autofluorescence, and Isotype Controls

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Description: When performing flow cytometry, you have to have the proper controls in order to understand what's real in your results. This month's episode of the Ken Lau show looks at the importance of autofluorescence, isotype controls, and fluorescence minus one controls. 

Links:

TruStain FcX™ Products


 

Episode 2 - Panel Building and Tiers

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Description: This month, we cover the fundamental building blocks useful for creating a panel. We discuss the proper controls that should be included with every experiment and how you can break your panel into easily digestible tiers for proper fluor assignment.

Links:

Fluorophore Brightness Chart
Rainbow Beads Blog
Multicolor Staining Guide
Panel Building Reference
Panel Builder
Tech Support


 

Episode 1 - Fluorescent Proteins

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Description: Fluorescent proteins produce fantastic colors and serve as excellent reporters for genes of interest. In our inaugural episode, Dr. Ken Lau takes you through the origin of the first fluorescent protein and how these proteins can affect your panel’s other markers.

Links:

GFP Blog
Spectra Analyzer
Multicolor Panel Selector
Custom Panel Construction 


 

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