Stem Cells and Development PosterStem cells are unique cells due to their capability to limitlessly self-renew and differentiate into each cell type in the adult body. Stem cells’ capability to differentiate depends on the stem cell type. Regardless of the type of stem cell you are working with, BioLegend offers stem cell-focused reagents for flow cytometry, cell screening, western blotting, ELISAs, cell differentiation, and more.

 

You can also download a copy of our Stem Cells and Development Poster for beautiful illustrations of the pathways tied to stem cell development.

Embryonic (ESCs) and Induced Pluripotent Stem Cells (iPSCs)

Embryonic stem cells (ESCs) are derived from the inner cell mass of the blastocyst and are capable of differentiating into each of the three primary germ layers: ectoderm, mesoderm, or endoderm. Due to the limited availability of human ESCs, researchers devised a method of generating pluripotent stem cells from adult cells through genetic reprogramming. These cells are referred to as induced pluripotent stem cells (iPSCs) and hold significant promise in the field of regenerative medicine and treatment of several diseases and disorders.

 

Browse products for ESCs and iPSCs

 

Hematopoietic Stem Cells (HSCs)

Hematopoietic stem cells (HSCs) are bone marrow-derived multipotent stem cells that give rise to all the blood cell types from the myeloid and lymphoid lineages including monocytes and macrophages, neutrophils, basophils, eosinophils, erythrocytes, megakaryocytes/platelets, dendritic cells, T cells, B cells, and NK cells.

 

Browse products for HSCs

Mesenchymal Stem Cells (MSCs)

Mesenchymal stem cells (MSCs) are multipotent stem cells derived from the mesoderm that can differentiate into a variety of cell types including: osteoblasts, chondrocytes, myocytes, adipocytes, and even β-pancreatic islet cells. MSCs are now widely studied to determine their possible utility in tissue engineering, wound healing, diabetes, and other conditions in which regenerated cells can improve or resolve disease.

 

Browse products for MSCs

 

Neural Stem Cells (NSCs)

Neural stem cells (NSCs) are multipotent cells which give rise to neurons, astrocytes, and oligodendrocytes of the central nervous system. Neural stem cells are being researched as potential therapeutics for several neurodegenerative diseases including Parkinson’s disease, Huntington’s disease, and multiple sclerosis.

 

Browse products for NSCs

Isolate – Separate your heterogeneous cell population with the MojoSort™ magnetic cell separation system or our comprehensive offering of FACS antibodies.
MojoSort™ | Flow Cytometry

 


 

Proliferate and differentiate – Utilize a wide range of cytokines, growth factors, and biofunctional antibodies to proliferate and differentiate your stem cells to various lineages and terminally differentiated cell types.
Recombinant Proteins | Ultra-LEAF™ Antibodies | GoInVivo™ Antibodies


 

 


Measure biomarkers​ – Measure stem cell markers in biological samples with ELISAs and LEGENDplex™ multiplex assays that can be preassembled or customized to include specific targets of interest.
ELISA | LEGENDplex™

 


 

 

Characterize signaling​ – Identify regulation of critical stem cell signaling pathways and transcription factors with highly sensitive Western-Ready™ western blotting reagents and antibodies.
Western-Ready™ Reagents and Antibodies

 

 

 

 

Phenotype – Explore a diversity of tools for phenotyping stem cells including an unmatched library of trusted flow cytometry antibodies, Maxpar® Ready antibodies that can be metal-labeled and used for CyTOF®, and TotalSeq™ oligonucleotide-conjugated antibodies for simultaneous proteome and transcriptome analysis.
TotalSeq | Flow Cytometry | Maxpar® Ready Antibodies

 


 

Visualize – Observe stem cell localization in tissue and identify cell types in mixed culture using a variety of primary and secondary antibodies for immunohistochemistry and immunocytochemistry. Utilize a wide-array of fluorophores including our Spark YG™ 570, Alexa Fluor® 488, Alexa Fluor® 594, Alexa Fluor® 647, Brilliant Violet 421™ and Brilliant Violet 510™ for comprehensive multicolor staining.
ICC and IHC Antibodies

In order to better phenotype stem cells at their various stages of development, it is important to know what markers they express (and don’t express) and having the right reagents at hand to detect them. This section gives a brief overview of common markers found on the various intermediate stages and indicates where markers may diverge between human and mouse equivalents. To see how these cells develop, view the full picture with our Stem Cell and Development Poster.

 

Embryonic Stem Cells
CD9CD24CD29CD49fCD117CD133CD324SSEA-1SSEA-3SSEA-4Tra-1-60Tra-1-81

Ectoderm
CD15CD29CD49fCD133GFAPNestinNeurofilament, Olig2, Pax6SOX2

Endoderm CXCR4FOXA2,  GATA4, GATA6, SOX17
 

Mesoderm
CD10CD16/32CD34CD38CD45RACD48CD90CD117CD123CD127CD133CD135CD150Sca-1
 

Hemangioblasts
Human: CD133
Mouse: Brachyury, CD135

 

Multipotent Progenitor
Human: CD34CD90CD135Lin-
Mouse: CD34-CD48-CD117CD150Sca-1  

 

Common Lymphoid Progenitor
(Differentiates into B cells, T cells, and ILC)
Human: CD10CD34CD45RACD135Lin- 
Mouse: CD117lowCD127CD135Lin-

 

Granulocyte-Monocyte Progenitor
(Differentiates into Monocytes, Macrophages, Dendritic Cells, and Granulocytes)
Human: CD34CD38CD115CD116CD123 
Mouse: CD16/32CD34CD117Sca-1
 

Hematopoietic Stem Cells
Human: CD34CD90CD135Lin-
Mouse: CD34-CD48-CD117CD150Sca-1  

 

Common Myeloid Progenitor
Human: CD34CD38CD45RA-CD135 
Mouse: CD34CD117CD135Sca-1-   

 

Megakaryocyte-Erythrocyte Progenitor
(Differentiates into RBCs and Megakaryocytes)
Human: CD34CD71CD110 
Mouse: CD34-CD41CD105CD150
 

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