Ultra-LEAF™ Purified anti-mouse CD115 (CSF-1R) Antibody

Pricing & Availability
AFS98 (See other available formats)
Regulatory Status
Other Names
CSF-1R, M-CSFR, c-fms
Rat IgG2a, κ
Ave. Rating
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Product Citations
Cat # Size Price Save
135537 100 µg ¥17,330
135538 1 mg ¥23,470
135539 5 mg ¥84,900
135540 25 mg ¥221,760
135541 50 mg ¥370,760
135542 100 mg ¥532,830

This product is eligible for a 40% discount! Promotion valid until December 31st, 2022. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.


CSF-1R, also known as CD115 and M-CSFR, is a single-pass type I membrane protein and member of the platelet-derived growth factor receptor family. This c-fms (Fms proto-oncogene) gene product's natural ligands include M-CSF and IL-34. Structural studies of CD115 have described an Ig-like extracellular domain, a transmembrane domain, an intracellular juxtamembrane domain, a split tyrosine kinase domain, and a C-terminal tail receptor. Receptor activation induces homodimerization in addition to phosphorylation and ubiquitination of intracellular residues. CD115 directly influences tissue macrophage and osteoclast differentiation and proliferation. It is expressed on monocytes/macrophages, peritoneal exudate cells, plasmacytoid and conventional dendritic cells, and osteoclasts.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Antibody Type
Host Species
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.

FC - Quality tested
Block - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: blocking of ligand binding1. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays.

It has been reported that CD115 can be rapidly internalized, especially when samples are exposed to room temperature. Approximate 33% decrease in CD115 expression has been observed between 0 and 4 hours after sample collection, while overnight incubation of the cells results in complete CD115 downregulation. Pre-treatment with EDTA and low temperatures (2 to 8°C) helps in maintaining surface expression of CD1154.

Application References

(PubMed link indicates BioLegend citation)
  1. Sudo T, et al. 1995. Oncogene 11:2469.
  2. Murayama T, et al. 1999. Circulation 99:1740.
  3. Jaeger BN, et al. 2012. J. Exp. Med. 209:565. PubMed
  4. Breslin WL, et al. 2013. J Immunol Methods. 390(1-2):1 PubMed
  5. Dong L, et al. 2016. Nature. 539:304–308. PubMed
AB_2832481 (BioLegend Cat. No. 135537)
AB_2832482 (BioLegend Cat. No. 135538)
AB_2832483 (BioLegend Cat. No. 135539)
AB_2832484 (BioLegend Cat. No. 135540)
AB_2832485 (BioLegend Cat. No. 135541)
AB_2832486 (BioLegend Cat. No. 135542)

Antigen Details

Ig superfamily, 145 kD

Monocytes/macrophages, peritoneal exudate cells, plasmacytoid and conventional dendritic cells, osteoclasts

Growth factor receptor, tyrosine kinase
Macrophage colony stimulating factor (M-CSF), IL-34
Cell Type
Dendritic cells, Macrophages, Monocytes, Osteoclasts
Biology Area
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References
  1. Sudo T, et al. 1995 Oncogene 11:2469.
  2. Murayama T, et al. 1999 Circulation 99:1740.
  3. Goswami S, et al. 2005 Cancer Res. 65:5278.
  4. Yu W, et al. 2008 J. Leuko. Biol. 84:852.
Gene ID
12978 View all products for this Gene ID
View information about CD115 on UniProt.org

Related FAQs

Why do I have a weak CD115 staining?

It has been reported that CD115 can be rapidly internalized, especially when samples are exposed to room temperature. Approximate 33% decrease in CD115 expression has been observed between 0 and 4 hours after sample collection, while overnight incubation of the cells results in complete CD115 downregulation.  Pre-treatment with EDTA and low temperatures (2 - 8°C) helps in maintaining surface expression of CD115. In addition, brief fixation of the cells with Fixation Buffer (Cat. No. 420801) for 10 minutes will block CD115 internalization.

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

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For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-mouse CD115 (CSF-1R)

  • PE anti-mouse CD115 (CSF-1R)

  • Biotin anti-mouse CD115 (CSF-1R)

  • APC anti-mouse CD115 (CSF-1R)

  • Alexa Fluor® 488 anti-mouse CD115 (CSF-1R)

  • Brilliant Violet 421™ anti-mouse CD115 (CSF-1R)

  • Brilliant Violet 605™ anti-mouse CD115 (CSF-1R)

  • Brilliant Violet 711™ anti-mouse CD115 (CSF-1R)

  • Alexa Fluor® 594 anti-mouse CD115 (CSF-1R)

  • Purified anti-mouse CD115 (CSF-1R) (Maxpar® Ready)

  • PE/Cyanine7 anti-mouse CD115 (CSF-1R)

  • PerCP/Cyanine5.5 anti-mouse CD115 (CSF-1R)

  • PE/Dazzle™ 594 anti-mouse CD115 (CSF-1R)

  • Alexa Fluor® 647 anti-mouse CD115 (CSF-1R)

  • APC/Cyanine7 anti-mouse CD115 (CSF-1R)

  • TotalSeq™-A0105 anti-mouse CD115 (CSF-1R)

  • APC/Fire™ 750 anti-mouse CD115 (CSF-1R)

  • Ultra-LEAF™ Purified anti-mouse CD115 (CSF-1R)

  • TotalSeq™-B0105 anti-mouse CD115 (CSF-1R)

  • TotalSeq™-C0105 anti-mouse CD115 (CSF-1R)


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