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Ultra-LEAF™ Purified anti-human/mouse TGF-β1 Antibody

Pricing & Availability
Clone
19D8 (See other available formats)
Regulatory Status
RUO
Other Names
Transforming growth factor-beta 1 (TGF-b1), TGFB1, CED, DPD1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
19D8_ULEAF_TGFb1_Antibody_1_62922
Recombinant human TGF-β1 (black circles, Cat. No. 781802) inhibits the proliferation of HT-2 cells induced by recombinant mouse IL-4 at 5 ng/mL (Cat. No. 574302). Ultra-LEAF™ Purified anti-human/mouse TGF-β1 antibody (purple circles, clone 19D8) inhibits the effect of recombinant human TGF-β1 (at 0.4 ng/mL) in mouse HT-2 cells in a dose-dependent manner; whereas the mouse IgG1, κ isotype control (grey triangles, clone MOPC-21, Cat. No. 400165) does not have effect. The ND50 = 0.04 – 0.2 µg/mL.
  • 19D8_ULEAF_TGFb1_Antibody_1_62922
    Recombinant human TGF-β1 (black circles, Cat. No. 781802) inhibits the proliferation of HT-2 cells induced by recombinant mouse IL-4 at 5 ng/mL (Cat. No. 574302). Ultra-LEAF™ Purified anti-human/mouse TGF-β1 antibody (purple circles, clone 19D8) inhibits the effect of recombinant human TGF-β1 (at 0.4 ng/mL) in mouse HT-2 cells in a dose-dependent manner; whereas the mouse IgG1, κ isotype control (grey triangles, clone MOPC-21, Cat. No. 400165) does not have effect. The ND50 = 0.04 – 0.2 µg/mL.
Cat # Size Price Save
521707 100 µg ¥39,350
521708 1 mg ¥123,340
Description

TGF-β1 is a multifunctional cytokine that plays pivotal roles in diverse biological processes. TGF-β1 is synthesized as a 390 amino acid precursor that is cleaved by furin, localized in the trans-Golgi network, in residue 278.  Furin processes the TGF-β1 precursor at the carboxyl side of the consensus sequence RHRR which precedes the NH2-terminal Ala 279 residue of the mature TGF-β1. The TGF-β1 precursor includes the latency-associated peptide (LAP dimer) in the N-terminal portion and the 25 kD portion that constitutes the mature TGF-β1 in the C-terminal. LAP dimer and the TGF-β1 mature protein remain non-covalently associated after furin cleavage and this complex does not bind to the TFG-β1 receptor. In addition, the TGF-β1 latent complex is joined covalently through LAP to LTBP. The TGF-β1 active form requires dissociation from LAP. Some activators can release TGF-β1 from LAP such as thrombospondin-1, reactive oxygen species, and the integrins avb6 and avb8.

Mouse TGF-β1 converts naïve T cells into regulatory T (Treg) cells that prevent autoimmunity. Although human TGF-β1 is widely used for inducing FOXP3+ in vitro, it might not be an essential factor for human Treg differentiation. Th17 murine can be induced from naïve CD4+ T cells by the combination of TGF-β1 and IL-6 or IL-21. Nevertheless, the regulation of human Th17 differentiation is distinct. TGF-β1 seems to have dual effects on human Th17 differentiation in a dose-dependent manner. While TGF-β1 is required for the expression of RORgt, in human naïve CD4+ T cells from cord blood, TGF-β1 can inhibit the function of RORgt at high doses. By using serum-free medium, it has been clarified that the optimum conditions for human Th17 differentiation are TGF-β1, IL-1b, and IL-2 in combination with IL-6, IL-21, or IL-23.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human TGF-β1
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Block - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by blocking activity of human TGF-β1 (500 pg/ml) in Mv1Lu-(caga)12-Luc reporter cells. The ED50 is 0.05 - 0.1 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Detection: To measure TGF-ß1, this antibody can be used as a detection antibody in sandwich ELISA format, and paired with the purified 21C11 antibody (Cat. No. 525301) as the capture antibody. Recombinant Human TGF-ß1 (ELISA Std.) (Cat. No. 580709) can be used as the protein standard.

Blocking: Clone 19D8 LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is useful for in vitro blocking of human and mouse TGF-ß1 bioactivity. The 19D8 clone does not inhibit the bioactivity of human TGF-ß2 or TGF-ß3 in the Mv1Lu-(caga)12-Luc reporter cell assay.

Note: For testing TGF-ß1 in serum, plasma or supernatant, BioLegend's ELISA LEGEND MAX™ Kits (Cat. No. 436708 & 437708) are specially developed and recommended.

Product Citations
  1. Li L, et al. 2021. Sci Adv. 7: . PubMed
  2. Nakayama K, et al. 2020. Cell Immunol. 358:104237. PubMed
  3. Yoon H, et al. 2021. Oncogenesis. 10:13. PubMed
  4. Weinstock A, et al. 2021. Elife. :10. PubMed
  5. Mansouri S, et al. 2020. Mucosal Immunol. 0.954861111. PubMed
  6. Shen Y, et al. 2022. Nat Commun. 13:3419. PubMed
  7. Rivera CA, et al. 2022. Immunity. 55:129. PubMed
RRID
AB_2810653 (BioLegend Cat. No. 521707)
AB_2810654 (BioLegend Cat. No. 521708)

Antigen Details

Structure
Homodimer
Distribution

TGF-β1 is secreted by numerous cells.

Function
TGF-β1 influences the fate of T cells into different subpopulations depending on the occurrence of other cytokines. TGF-β1 induces cell growth and survival, cell and tissue differentiation, development, inflammation, immunity, hematopoiesis, tissue remodeling, wound healing, and stimulates ECM deposition and angiogenesis. It is an essential mediator of pathologic scarring in fibrotic disorders.
Interaction
Multiple cells express the TGF-β1 receptors.
Ligand/Receptor
Heterodimeric receptor consisting of type I (TbRI) and type II (TbRII).
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References

1. Dubois CM, et al. 1995. J. Biol. Chem. 270:10618.
2. Dennler S, et al. 1998. EMBO J. 17:3091.
3. Annes JP, et al. 2003. J. Cell. Sci. 116:217.
4. Zou Z and Sun PD. 2004. Protein Expr. Purif. 37:265.
5. Park SH, 2005. J. Biochem. Mol. Biol. 38:9.
6. Valcourt U, et al. 2005. Mol. Biol. Cell. 16:1987.
7. Mucida D, et al. 2007. Science 317:256.
8. Acosta-Rodriguez EV, et al. 2007. Nat. Immunol. 8:942.
9. Puthawala K, et al. 2008. Am. J. Respir. Crit. Care Med. 177:82.
10. Takatori H, et al. 2008. Mod. Rheumatol. 18:533.
11. Manel N, et al. 2008. Nat. Immunol. 9:641.
12. Veldhoen M, et al. 2008. Nat. Immunol. 9:1341.
13. Oida T, et al. 2011. PLoS ONE 6:e18365.

Gene ID
7040 View all products for this Gene ID 21803 View all products for this Gene ID
UniProt
View information about TGF-beta1 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 2    Revision Date: 06/29/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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