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Spark Red™ 718 anti-human CD27 Recombinant Antibody

Pricing & Availability
Clone
QA17A18 (See other available formats)
Regulatory Status
RUO
Other Names
S152, T14, TNFRSF7
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
QA17A18_SparkRed718_CD27_RECOM_Antibody_071822
Human peripheral blood lymphocytes were stained with anti-human CD3 FITC and anti-human CD27 recombinant (clone QA17A18) Spark Red™ 718 (left) or stained with anti-human CD3 FITC only (right).
  • QA17A18_SparkRed718_CD27_RECOM_Antibody_071822
    Human peripheral blood lymphocytes were stained with anti-human CD3 FITC and anti-human CD27 recombinant (clone QA17A18) Spark Red™ 718 (left) or stained with anti-human CD3 FITC only (right).
Compare all formats
Cat # Size Price Save
393217 25 tests ¥26,250
393218 100 tests ¥58,800
Description

CD27 is a 50-55 kD type I membrane protein also known as S152 and T14. It is a lymphocyte-specific member of the TNF-receptor superfamily. CD27 is expressed on medullary thymocytes, virtually all mature T cells, some B cells, and NK cells. CD27 binds to CD70, and plays a role in costimulation of T cell activation and regulation of B cell differentiation and proliferation. The cytoplasmic domains of CD27 have also been shown to interact with TRAF2 and TRAF5 to elicit NF-κB and SAPK/JNK activation.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Recombinant
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Spark Red™ 718 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark Red™ 718 has a maximum excitation of 697 nm and a maximum emission of 718 nm.

Excitation Laser
Red Laser (633 nm)
Application Notes

Clone QA17A18 does not block clones 0323, M-T271, or LG.3A10, indicating a unique epitope.

RRID
AB_2924589 (BioLegend Cat. No. 393217)
AB_2924589 (BioLegend Cat. No. 393218)

Antigen Details

Structure
TNF-R superfamily, type I transmembrane glycoprotein.
Distribution

Medullary thymocytes, T and B cell subsets, NK cells.

Ligand/Receptor
CD70
Cell Type
B cells, NK cells, T cells, Thymocytes
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules, TCRs
Antigen References
  1. Knapp W, et al. 1989. Immunol. Today 10:253-8
  2. Schlossman S, et al. 1995. Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press.
  3. Hintzen R, et al. 1994. Immunol. Today 15:307.
  4. Agematsu K, et al. 1995. J. Immunol. 154:3627.
Gene ID
939 View all products for this Gene ID
UniProt
View information about CD27 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 07/18/2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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