Recombinant Mouse IL-5 (carrier-free)

Pricing & Availability
Other Names
Interleukin 5
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Product Citations
publications
IL-5_Mouse_033012
BCL-1 cell proliferation induced by mouse IL-5.
  • IL-5_Mouse_033012
    BCL-1 cell proliferation induced by mouse IL-5.
Cat # Size Price Save
581502 10 µg ¥20,700
581504 25 µg ¥34,200
581506 100 µg ¥103,500
581508 500 µg ¥306,000
Description

IL-5 is a homodimeric glycoprotein that was initially identified by its ability to support the in vitro growth and differentiation of mouse B cells and eosinophils. IL-5 induces eosinophil progenitor cell proliferation, terminal differentiation, and activation. In animal models of allergic diseases or helminth infection, IL-5 induces a massive proliferation of eosinophil progenitors in the bone marrow, promotes eosinophil recruitment with eotaxins, and prolongs eosinophil survival in local tissues. IL-5 regulates genes involved in the B cell terminal differentiation. IL-5 induces CD38-activated splenic B cells to differentiate into immunoglobulin M-secreting cells and go through m to g1 class switch recombination at the DNA level, resulting in immunoglobulin G1 (IgG1) production. IL-5 binds the IL-5R complex, which consists of an IL-5Rα chain specific for IL-5 and a common β-chain that is shared by the receptors for IL-3 and GM-CSF. The alpha subunit is required for ligand-specific binding whereas association with the beta subunit results in increased binding affinity. IL-5 plays important roles in the pathogenesis of asthma, hypereosinophilic syndromes, and eosinophil-dependent inflammatory disease.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Source
Mouse IL-5, amino acids Met21-Gly133 (NM_010558.1), was expressed in insect cells.
Molecular Mass
The 113 amino acid recombinant protein has a predicted molecular mass of approximately 13.1 kD. The DTT-reduced protein migrates between 13 to 20 kD and the non-reduced protein at approximately 40 kD by SDS-PAGE. The N-terminal amino acid is Met.
Purity
>98%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS, pH 7.2.
Endotoxin Level
Less than 0.01 ng per µg cytokine as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Please note, new lots of the 100 µg size will be lot-specific and may differ from previous lots that had a fixed concentration.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
Bioactivity was measured by its property to stimulate the proliferation of BCL1 cells in a dose dependent manner. ED50 = 0.03 - 0.15 ng/ml, corresponding to a specific activity of 0.66 - 3.3 x 107 units/mg.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Product Citations
  1. Hatchwell L, et al. 2015. Thorax. 70: 854-861. PubMed
Publication Library

Antigen Details

Structure
Homodimeric cytokine
Distribution

Activated Th2 cells, mast cells, eosinophils, and basophils. In addition, newly identified IL-5 producing cells are: natural helper cells or nuocytes (lately identified as type II innate lymphoid cells), MPPtype2, and Ih2 cells.

Function
IL-5 regulates the production of eosinophils from purified hematopoieitic progenitors and regulates genes involved in the B cell terminal differentiation. IL-25 and IL-33 induce Th2 cytokines, among them IL-5.
Interaction
Eosinophils, B cells, basophils, and activated T cells.
Ligand/Receptor
Heterodimer IL-5Rα (CD125); β-subunit (CDw131) in common with IL-3R, GM-CSFR
Cell Type
Hematopoietic stem and progenitors, Embryonic Stem Cells
Biology Area
Cell Biology, Signal Transduction, Immunology, Stem Cells
Molecular Family
Cytokines/Chemokines
Antigen References

1. Lopez AF, et al. 1988. J. Exp. Med. 167:219.
2. Horikawa K and Takatsu K, 2006. Immunology 118:497.
3. Moro, K, et al. 2010. Nature 463:540.
4. Neill, DR, et al. 2010. Nature 464:1367.
5. Saenz SA, et al. 2010. Nature 464:1362.
6. Ikutai M, et al. 2012. J. Immunol. 188:703.
7. Yazuda K, et al. 2012. P. Nat. Acad. Sci. USA 109:3451.

Gene ID
16191 View all products for this Gene ID
UniProt
View information about IL-5 on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Go To Top Version: 3    Revision Date: 08/19/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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