- Other Names
- Tumor necrosis factor receptor superfamily member 4, TNFRSF4, ACT35, OX40L receptor, TAX transcriptionally-activated glycoprotein 1 receptor, CD134
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- Product Citations
OX40 (ACT35, CD134, TNFRSF4) is an approximately 48 kD transmembrane protein belonging to the TNF receptor superfamily. It is a 249 amino acid glycoprotein with a 41 amino acid cytoplasmic tail, 20 amino acid transmembrane domain, and a 185 amino acid extracellular domain. OX40 is a costimulatory molecule implicated in long-term T cell immunity. It is a receptor for TNFSF4, OX40L, GP34, and also acts as a receptor for human herpesvirus 6B/HHV-6B. OX40 is primarily expressed on activated T cells, including CD4 and CD8 T cells, Th1, Th2, and Th17 cells, and regulatory T cells. The main source of OX40L is antigen presenting cells. OX40L can also be induced on activated B cells, mature dendritic cells, Langerhans cells, and macrophages. OX40 and OX40L interactions regulate the division and survival of T cells. It inhibits the conversion of effector T cells into regulatory T cells (Tregs) and promote the recall response in memory T cells. OX40 and OX40L interactions are also involved in the development of multiple diseases including allergic airway inflammation, graft-versus-host disease and autoimmune disease.Product Details
- Human OX40/TNFRSF4, amino acid Leu29-Ala214 (Accession # P43489) with a C-terminal human IgG Fc tag was expressed in 293E cells.
- Molecular Mass
- The 425 amino acid recombinant protein has a predicted molecular mass of approximately 46.9 kD. The DTT-reduced proteins migrate at approximately 65 kD, and non-reduced proteins migrate at approximately 130 kD by SDS-PAGE. The predicted N-terminal amino acid is Leu.
- > 95%, as determined by Coomassie stained SDS-PAGE.
- 0.22 μm filtered protein solution is in PBS, pH 7.2.
- Endotoxin Level
- Less than 0.1 EU per μg protein as determined by the LAL method.
- 10-25 µg sizes are bottled at 200 µg/mL. 100 µg size is lot-specific and bottled at the concentration indicated on the vial. (Please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- When human OX40L (Cat. No. 555704) is immobilized at 1.0 µg/mL, human OX40/TNFRSF4-Fc Chimera binds with EC50 of 20 - 80 ng/mL. HRP Protein A (Cat. No. 689202) was used to detect the binding.
- Application Notes
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at email@example.com.
- Disulfide linked homodimer
Primarily expressed on the activated T cells, including CD4 and CD8 T cells, Th1, Th2, and Th17 cells, and regulatory T cells.
- Is a costimulatory molecule implicated in long-term T-cell immunity
- TNFSF4, OX40L, GP34, human herpesvirus 6B/HHV-6B.
- Measured by its ability to bind OX40L
- Cell Targets
- Antigen presenting cells
- Cell Type
- B cells, T cells, Th1, Th17, Th2, Tregs
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Immunology
- Molecular Family
- Immune Checkpoint Receptors, Soluble Receptors
- Antigen References
- Baum PR, et al. 1994. Circ. Shock. 44:30-4.
- Bansal-Pakala P, et al. 2001. Nat. Med. 7:907-12.
- Humphreys IR, et al. 2003. J. Exp. Med. 198:1237-42.
- Shimojima M, et al. 2004. Science. 303:1192-5
- Tang H, et al. 2013. Proc. Natl. Acad. Sci. U.S.A. 110:9096-9.
- Jacquemin C, et al. 2015. Immunity 42:1159-70.
- Gene ID
- 7293 View all products for this Gene ID
- View information about OX40/TNFRSF4 on UniProt.org
- Does specific activity of a recombinant protein vary between lots?
Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- Have your recombinants been tested for stability?
Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
- Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
- How does the activity of your recombinant proteins compare to competitors?
We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.