Recombinant Human IL-1α (ELISA Std.)

Pricing & Availability
Other Names
interleukin 1, alpha, IL-1A, IL1F1
Ave. Rating
Submit a Review
Product Citations
publications
IL-1alpha_Human_ELISA_111313
  • IL-1alpha_Human_ELISA_111313
Cat # Size Price Save
570009 4 pack ¥14,400
Description

IL-1 was isolated from human blood that had been exposed to a pathogenic bacterium. IL-1 is a pyrogen, and it is an activating factor for lymphocytes. It also damaged joints and influenced liver proteins (2). IL-1α binds to the cell surface type I and II IL-1 receptors (IL-1RI and IL-1RII). IL-1 and -β and IL-1RA can compete for binding to these receptors. However, only IL-1RI, not IL-1RII, is functional because IL-1RII lacks a cytoplasmic domain and is thus unable to transmit signals to downstream steps (3). IL-1α is expressed by cancer cells and promotes angiogenesis and metastasis of pancreatic cancer and human gastric cancer cell lines (4). In addition, IL-1 induced the expression of VEGF in colon cancer (5).

Product Details
Technical data sheet

Product Details

Reactivity
Human
Source
Human IL-1α, amino acids Ser113-Ala271 (Accession # X03833) was expressed in E. coli, approximately 18 kD
Formulation
Lyophilized in sterile-filtered PBS, pH 7.2, containing 1% BSA, 0.09% sodium azide, and protease inhibitors.
Preparation
ELISA Standard 4-Pack contains four vials of recombinant human IL-1α at >5 ng per vial. Recommended for ELISA application only. Standards are calibrated against a BioLegend Master Calibrator.
Storage & Handling
Upon receipt, store unopened vials between 2°C and 8°C immediately and use within 12 months from date of receipt. Prior to use, reconstitute the lyophilized powder with 0.2 ml of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70°C for up to one month. Do not re-use diluted standards. Use a manual defrost freezer and avoid repeated freeze thaw cycles.
Application

ELISA

Application Notes

This IL-1α protein is useful as a standard for a human IL-1α sandwich ELISA, using unlabeled 364-3B3-14 antibody (catalog #500104) for capture and biotinylated Poly5313 antibody (catalog #531304) for detection.

Antigen Details

Structure
Cytokine
Distribution

IL-1α is produced by monocytes, macrophages, keratinocytes, stimulated B lymphocytes, fibroblasts, endothelial cells, and epithelial cells.

Function
The cytokine IL-1α is a key molecule both in the innate and in the adaptive immune response. IL-1α induces autonomous proliferation in vascular smooth muscle cells, T cell activation during Ag presentation, up-regulation of monocyte/macrophage-mediated tumor cytotoxicity, stimulation of osteoclast formation, and production of proteinases in human chondrocytes (1).
Ligand/Receptor
Type I and II IL-1 receptors (IL-1RI and IL-1RII)
Biology Area
Stem Cells, Immunology, Cell Biology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Yatabe T, et al. 2008. Ann Rheum Dis
2. Ledford H, et al. 2007. Nature 450
3. Boraschi B Tagliabue A, et al. 2006. Vitam Horm 74:229
4. Shao J and Sheng H, et al. 2007. J. Immunol. 178:4097
5. Elaraj DM, et al. 2006. Clin Cancer Res 12:1088

Gene ID
NA
UniProt
View information about IL-1alpha on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 02/22/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

Login / Register
Forgot your password? Reset password?
Create an Account