- Other Names
- Colony stimulating factor 2, CSF2, Granulocyte/macrophage colony-stimulating factor, CSF-α, Pluripoietin-α, Eosinophil colony stimulating factor (Eo-CSF), Burst promoting activity (BPA).
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- Product Citations
GM-CSF plays a key role in signaling emergency hemopoiesis (predominantly myelopoiesis) in response to infection, including the production of granulocytes and macrophages in the bone marrow and their maintenance, survival, and functional activation at sites of injury or insult. The receptor for GM-CSF is a heterodimer that comprises a major binding subunit (GMRα) and a major signaling subunit (βc). The receptor subunits are always coexpressed on the surface of leukocytes, with βc being expressed at lower levels than GMRα. Certain nonhemopoietic cell types have also been reported to express the GM-CSF receptor and to respond to GM-CSF stimulation in vitro, although the in vivo significance of these observations remains uncertain.Product Details
- Human GM-CSF, amino acids Ala18- Glu144 (Accession# AAA52578) was expressed in E. coli, approximately 14.6 kD.
- Lyophilized in sterile-filtered PBS, pH 7.2, containing 1% BSA, 0.09% sodium azide, and protease inhibitors.
- ELISA Standard 4-Pack contains four vials of recombinant human GM-CSF at >5 ng per vial. Recommended for ELISA application only. Standards are calibrated against a BioLegend Master Calibrator.
- Storage & Handling
- Upon receipt, store unopened vials between 2°C and 8°C immediately and use within 12 months from date of receipt. Prior to use, reconstitute the lyophilized powder with 0.2 ml of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70°C for up to one month. Do not re-use diluted standards. Use a manual defrost freezer and avoid repeated freeze thaw cycles.
- Recommended Usage
Each lot of this protein is quality control tested by ELISA assay. For use as an ELISA standard, a standard curve comprised of doubling dilutions from 500 pg/ml to 4 pg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
This GM-CSF protein is useful as a standard for a human GM-CSF sandwich ELISA, using unlabelled anti-GM-CSF antibody, clone BVD2-23B6 (catalog #502202) for capture and biotinylated BVD2-21C11 (catalog #502304) antibody for detection.
T cells, macrophages, fibroblasts, endothelial cells, mast cells.
- Synergistic with IL-1, IL-3, G-CSF; E21R competitive antagonist for receptor binding; stored in ECM with heparan sulfate proteoglycans
- Granulocyte/macrophage/erythroid/megakaryocytic progenitors, myeloblasts, monoblasts, dendritic cells, T cells.
- Heterodimer GM-CSFR α subunit (CDw116); β-subunit (CDw131) in common
- Granulocyte-macrophage colony-stimulating factor (GM-CSF) and the related cytokines interleukin (IL)—3 and IL-5 regulate the production and functional activation of hemopoietic cells, with GM-CSF acting on monocyte/macrophages and all granulocytes. GM-C
- Biology Area
- Cell Biology, Stem Cells
- Molecular Family
- Growth Factors, Cytokines/Chemokines
- Antigen References
1. Hercus TR, et al. 2009 Blood 114:1289-1298.
2. Hayashida K, et al. 1990 Proc Natl Acad Sci USA. 87:9655-9659.
3. Walker F, et al. 1985 EMBO J 4:933-939.
4. Kitamura T, et al. 1989 J. Cell Physiol. 140:323.
- Gene ID
- View information about GM-CSF on UniProt.org