- Other Names
- Low affinity immunoglobulin gamma Fc region receptor II-A, Fc fragment of IgG receptor IIa, IgG Fc receptor II-a, CDw32, Fc-gamma RII-a, FcγRII-a, FcRII-a, CD32a, FCGR2A
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- Product Citations
Fcγ RII, also known as CD32, is a low affinity IgG Fc receptor. There are three distinct genes encoded (A, B, C) in humans and a single gene in mice. Fcγ RIIA, also known as CD32a, is an immunoglobulin superfamily member.
Fcγ RIIA consists of an extracellular domain, a transmembrane region and a cytoplasmic tail containing a non-classical ITAM, which appears to be required for Fcγ RIIA dimerization and downstream intracellular signaling. Fcγ RIIA is the most widespread activating Fc receptor and it is expressed on monocytes/macrophages, granulocytes, platelets and mast cells in humans. Engagement of Fcγ RIIA and IgG induces inflammatory reactions and they are involved in the pathophysiology of several diseases, such as thrombocytopenia, rheumatoid arthritis and systemic lupus erythematosus. Fcγ RIIA is the only FcR expressed on platelets and cross-linking of Fcγ RIIA by antibodies activates platelets for aggregation and secretion. Unlike Fcγ RI and Fcγ RIIIA, the expression and phagocytic activity of Fcγ RIIA is independent of the Fc γ-chain. It has been reported that Fcγ RIIA associates with lipid rafts which regulates its ligand binding activity. The affinities of Fcγ RIIA for different IgG subclasses vary. In addition, R167H polymorphism affects the binding affinity of Fcγ RIIA to IgG2, but hardly affects the affinity for IgG1, IgG3, and IgG4. It has been reported that Fcγ RIIA is highly induced in HIV-infected quiescent CD4 T cells, serving as a biomarker.
- Human Fcγ RIIA/CD32a, amino acid Ala36-Ile218 (Accession # AAA35827.1), with a C-terminal 10x His tag, was expressed in 293E cells.
- Molecular Mass
- The 193 amino acid recombinant protein has a predicted molecular mass of approximately 21.7 kD. The DTT-reduced and non-reduced protein migrates at approximately 33 kD by SDS-PAGE. The predicted N-terminal amino acid is Ala.
- >95%, as determined by Coomassie stained SDS-PAGE.
- 0.22 µm filtered protein solution is in PBS, pH 7.2.
- Endotoxin Level
- Less than 0.01 ng per µg cytokine as determined by the LAL method.
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year. For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 50-100 µg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 0.2 - 1% BSA or HSA and stored in working aliquots at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
- When human Fcγ RIIA/CD32a is coated at 2 µg/mL (100 μL/well), human IgG1 Fc (Cat. No. 778302) binds with an EC50 of 0.05 – 0.2 µg/mL in a functional ELISA.
- Application Notes
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at firstname.lastname@example.org
- Ig family molecule
Myeloid cells, monocytes, macrophages, dendritic cells, neutrophils, eosinophils and platelets; Plasma membrane
- Low affinity IgG Fc receptor, It is involved in phagocytosis, cytotoxicity, platelet aggregation and secretion
- Aggregated IgG, IgG-antigen complex
- Cell Type
- Dendritic cells, Eosinophils, Macrophages, Mast cells, Monocytes, Neutrophils, Platelets
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules, Fc Receptors, Soluble Receptors
- Antigen References
- Bruhns P, et al. 2009. Blood 113: 3716.
- Bewarder N, et al. 1996. Mol Cell Biol. 16: 4735.
- Descours B, et al. 2017. Nature 543: 564.
- Tomiyama Y, et al. 1992. Blood 80: 2261.
- Indik Z, et al. 1991. J Clin Invest. 88: 1766.
- Ramsland PA, et al. 2011. J Immunol. 187: 3208.
- Hogarth PM and Pietersz GA. 2012. Nat Rev Drug Discov. 11: 311.
- Bournazos S, et al. 2009. J Immunol. 182: 8026.
- Maxwell KF, et al. 1999. Nat Struct Biol. 6: 437.
- Sandilands GP, et al. 1997. Immunology 91: 204.
- Ghazizadeh S, et al. 1994. J Biol Chem. 269: 8878.
- Gillis C, et al. 2014. Front Immunol. 5: 254.
- Gene ID
- 2212 View all products for this Gene ID
- View information about Fcgamma RIIA on UniProt.org
- Does specific activity of a recombinant protein vary between lots?
Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- Have your recombinants been tested for stability?
Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
- Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
- How does the activity of your recombinant proteins compare to competitors?
We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.