Recombinant Human Cystatin C (carrier-free)

Pricing & Availability
Other Names
Cystatin-3, Gamma-trace, Neuroendocrine basic polypeptide, Post-gamma-globulin, gamma-CSF, post-gamma protein
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CystatinC_CF_Human_RECOM_BA_033016
The activity of human Cystatin C was measured by its ability to inhibit papain activity. The papain activity was monitored by the cleavage of a fluorogenic substrate, Z-FR-AMC, with 50 ng/mL of activated papain. The recombinant human Cystatin C displays a value of IC50 < 6 nM.
  • CystatinC_CF_Human_RECOM_BA_033016
    The activity of human Cystatin C was measured by its ability to inhibit papain activity. The papain activity was monitored by the cleavage of a fluorogenic substrate, Z-FR-AMC, with 50 ng/mL of activated papain. The recombinant human Cystatin C displays a value of IC50 < 6 nM.
Cat # Size Price Save
756202 10 µg ¥23,400
756204 25 µg ¥45,000
756206 100 µg ¥130,500
756208 500 µg ¥387,000
Description

Cystatin C was initially identified in human urine and was named gamma-trace. It is a type II cystatin sub-family member. The cystatin family contains three subfamilies: Type 1 cystatins, also known as stefins, which are predominantly present in the cytosol and the nuclei; Type 2 cystatins are mainly secreted proteins; Type 3 cystatins are multi-domain proteins of high molecular weight (60-120 kD) and are also known as kininogens. Cystatin C inhibits cysteine proteases from the papain family and legumain. It forms tight non-covalent associations with its target enzymes. Cystatin C antagonizes TGF-β signaling in normal and cancer cells in vitro. In this process, Cystatin C binds to the TGF-β type II receptor and inhibits the binding of TGF-β. Cystatin C influences the progression of diseases such as, cancer metastasis, atherosclerosis, aortic aneurisms, emphysema, and arthritis. Also, it is associated with neurodegenerative disease and cerebral angiopathy. Human Cystatin C has the tendency to dimerize in order to produce very tight two-fold symmetric dimers. This amyloidogenic property suggests a mechanism for its aggregation in the brain arteries of elderly people with amyloid angiopathy. The L68Q mutant of human Cystatin C is responsible for conformational disease, which causes massive amyloidosis, cerebral hemorrhage, and death in young adults.

Product Details
Technical data sheet

Product Details

Source
Human Cystatin C, amino acids (Ser27-Ala146) (Accession# P01034), was expressed in 293E cells. The carboxi terminal contains a TG-10His-GGQ-tag.
Molecular Mass
The 135 amino acid recombinant protein has a predicted molecular mass of approximately 15.1 kD. The DTT-reducing and non-reducing protein migrates at approximately 17 kD by SDS-PAGE. The predicted N-terminal amino acid is Ser.
Purity
>95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein is in 20 mM HEPES, 0.1 M NaCl, and in pH 7.2.
Endotoxin Level
Less than 0.1 EU per µg cytokine as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Please note, new lots of the 100 µg size will be lot-specific and may differ from previous lots that had a fixed concentration.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
IC50 < 6.0 nM, as determined by inhibiting human Papain (0.05 µg/mL) activity when 100 uM of Z-FR-AMC is used as a papain peptide substrate.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Monomer.
Distribution

Considered ubiquitously expressed, biological fluids, dendritic cells, and macrophages.

Function
Inhibitor of cysteine proteases (capthepsins B, H, K, L, and S). Transcription factor IFN regulatory factor 8 (IRF-8) induces cystatin C in mouse primary dendritic cells. IL-10 inhibits IRF-8 and consequently Cystatin C.
Interaction
Normal and cancer cells.
Ligand/Receptor
Papain-like cysteine proteases, cathepsins B, H, K, L, and S, and legumain.
Bioactivity
Inhibits cystein proteases.
Biology Area
Cancer Biomarkers, Cell Biology
Molecular Family
Enzymes and Regulators
Antigen References

1. Grubb A, et al. 1982. Proc. Nat. Acad. Sci. 79:3024.
2. Janowski R, et al. 2001. Nat. Struct. Biol. 8:316.
3. Sokol JP, Schiemann WP. 2004. Mol. Cancer Res. 2:183.
4. Bird PI, et al. 2009. Nat Rev Immunol. 9:871.
5. Kaur G, Levy E. 2012. Front Mol. Neurosci. 5:79.
6. Xu Y, et al. 2014. J Biol Chem. 289:9730.
7. Xu Y, et al. 2015. Immunol. Cell Biol. 5:442-51.

Gene ID
1471 View all products for this Gene ID
UniProt
View information about Cystatin C on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Go To Top Version: 1    Revision Date: 03/31/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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