Recombinant Human CD31 (ELISA Std.)

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Other Names
PECAM-1, EndoCAM
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Human_CD31_RECOM_ELISA_032317
  • Human_CD31_RECOM_ELISA_032317
Cat # Size Price Save
566801 4 pack ¥14,400
Description

CD31 is a 130-140 kD type I transmembrane glycoprotein also known as platelet endothelial cell adhesion molecule-1 (PECAM-1) or Endocam. It is expressed on monocytes, platelets, granulocytes, endothelial cells and lymphocyte subsets. CD31 has been reported to bind CD38 and be involved in wound healing, angiogenesis, and cellular migration during inflammatory responses.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Source
HEK293 cells
Molecular Mass
The recombinant protein has a predicted molecular mass between 130-140 kD.
Purity
>95%, as determined by SDS-PAGE Analysis
Formulation
Lyophilized in sterile-filtered PBS, pH 7.2, containing 1% BSA, 0.09% sodium azide, and protease inhibitors.
Endotoxin Level
Less than 0.01 ng per µg cytokine as determined by the LAL method.
Preparation
ELISA Standard 4-Pack contains four vials of recombinant human CD31 at >5 ng per vial. Recommended for ELISA application only. Standards are calibrated against a BioLegend Master Calibrator.
Concentration
≥ 5ng/vial
Storage & Handling
Upon receipt, store unopened vials between 2°C and 8°C immediately and use within 12 months from date of receipt. Prior to use, reconstitute the lyophilized powder with 0.2 ml of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH 7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70°C for up to one month. Do not re-use diluted standards. Use a manual defrost freezer and avoid repeated freeze thaw cycles.
Activity
Determined by its ability to support the adhesion of activated Jurkat cells. The expected ED50 for this effect is 1.0 -1.5 µg/ml.
Application

ELISA - Quality tested

Recommended Usage

Each lot of this protein is quality control tested by ELISA. For use as an ELISA standard, a standard curve comprised of doubling dilutions from 10 ng/mL to 0.156 ng/mL is suggested. It is recommended that the reagent be titrated for optimal performance.

Application Notes

This CD31 protein is useful as a standard for a human CD31 sandwich ELISA, using unlabeled WM59 (Cat. No. 303102) antibody as capture and biotinylated O92E4 (Cat. No. 536604) antibody as detection.

Note: For testing human CD31 in cell culture supernatant, serum, plasma, other biological fluids, BioLegend's LEGEND MAX™ Kits (Cat. No. 443607 & 443608) are specially developed and recommended.

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 130-140 kD
Distribution

Monocytes, platelets, granulocytes, endothelial cells, lymphocyte subset

Function
Cell adhesion, signal transduction
Ligand/Receptor
CD38
Biology Area
Immunology
Molecular Family
Adhesion Molecules
Antigen References

1. DeLisser H, et al. 1994. Immunol. Today 15:490.
2. Newman P, 1997. J. Clin. Invest. 99:3.
3. Fawcett J, et al. 1995. J. Cell Biol. 128:1229.

Gene ID
5175 View all products for this Gene ID
UniProt
View information about CD31 on UniProt.org

Related FAQs

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Go To Top Version: 0    Revision Date: 03/27/2017

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