Rainbow Fluorescent Particles, 1 peak (3.0-3.4 µm) - Mid Range Intensity

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Other Names
Sphero™ brand beads, 1 peak Rainbow Calibration Bead
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RFP_1-peakMRI_1_080812
Bead fluorescence at 405 nm excitation with a 450/50 BP filter (Pacific Blue™ channel).
  • RFP_1-peakMRI_1_080812
    Bead fluorescence at 405 nm excitation with a 450/50 BP filter (Pacific Blue™ channel).
  • RFP_1-peakMRI_2_080812
    Bead fluorescence at 488 nm excitation with a 530/30 BP filter (FITC channel).
  • RFP_1-peakMRI_3_080812
    Bead fluorescence at 561 nm excitation with a 582/15 BP filter (PE channel).

    Rainbow Fluorescent Particles were analysed on a 4-laser BD LSRFortessa™ using detector voltages from a 15-color surface phenotyping assay identifying immune cell subsets in lysed whole blood.
Cat # Size Price Save
422905 5 ml ¥49,000
Description

These Rainbow Fluorescent Particles consist of uniformly sized particles dyed with a mixture of fluorophores. These particles produce a single peak with tight CV in each detection channel of conventional flow cytometers within the specified excitation range. Rainbow Fluorescent Particles can be used with most optical filters intended for analysis of fluorophores commonly used for flow cytometry. As the excitation range of these rainbow particles is 365 - 650 nm, they can be used with most lasers. However, they are not for use with the UV laser. The emission spectra of these rainbow particles is compatible with many common fluorophores used in immunofluorescent staining with flow cytometric analysis.

 

For standardization of cytometer detector settings between experiments, Rainbow Fluorescent Particles can be used to provide target channels to facilitate set up, ensuring data consistency in consequent runs.  Rainbow Fluorescent Particles are available in two intensity levels: Mid Range Intensity (Cat. No. 422905) and Bright Intensity (Cat. No. 422907), providing flexibility to accommodate samples with different levels of background fluorescence.

Product Details
Technical data sheet

Product Details

Formulation
Rainbow Fluorescent Particles are in deionized water with 0.02% sodium azide and 0.01% NP-40.
Concentration
107 particles per ml.
Storage & Handling
Store between 2°C and 8°C. Do not freeze. Protect from light.
Application

FC

Recommended Usage

Resuspend well (e.g. vortex briefly) before adding 3-5 drops to 1 ml of filtered DI water.

Application Notes

Use of Rainbow Fluorescent Particles, 1 peak (3.0-3.4 µm) - Mid Range Intensity, is common for the following purposes:
1. Laser alignment
2. Standardization of instrument settings for longitudinal studies (can also use Rainbow Fluorescent Particles, 1 peak, Bright Intensity (Cat. No. 422907), and multi-peak Rainbow Calibration Particles (Cat. No. 422901 and 422903)

Application References

(PubMed link indicates BioLegend citation)
  1. Perfetto SP, et al. 2006. Nat. Protoc. 1:1522.

Antigen Details

Gene ID
NA

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Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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