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Purified anti-TSC2 Antibody

Pricing & Availability
Clone
W16104A (See other available formats)
Regulatory Status
RUO
Other Names
Tuberin, Tuberous sclerosis 2 protein, TSC4
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W16104A_PURE_TSC2_Antibody_WB_1_051217
Total lysates (15µg protein) from HeLa, Jurkat and NIH3T3 cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with purified anti-TSC2 antibody (clone W16104A, 2µg/ml) (upper) or GAPDH (poly6314) antibody (lower). Proteins were visualized using an HRP goat anti-rat-IgG secondary antibody for TSC2 or HRP Donkey anti-rabbit IgG Antibody for GAPDH and chemiluminescence detection. Lane M is the molecular weight ladder and M* indicates extended exposure of the molecular weight ladder.
  • W16104A_PURE_TSC2_Antibody_WB_1_051217
    Total lysates (15µg protein) from HeLa, Jurkat and NIH3T3 cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with purified anti-TSC2 antibody (clone W16104A, 2µg/ml) (upper) or GAPDH (poly6314) antibody (lower). Proteins were visualized using an HRP goat anti-rat-IgG secondary antibody for TSC2 or HRP Donkey anti-rabbit IgG Antibody for GAPDH and chemiluminescence detection. Lane M is the molecular weight ladder and M* indicates extended exposure of the molecular weight ladder.
  • W16104A_PURE_TSC2_Antibody_WB_2_051217
    Total lysates (15µg protein) from HeLa (Lane 1), 5nM (Lane 2)and 20nM (Lane 3) TSC2 siRNA treated HeLa cells were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified 1µg/mL anti-TSC2 antibody (upper). Proteins were visualized using an HRP goat anti-rat-IgG secondary antibody and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin Antibody was used as a loading control (lower).
Cat # Size Price Save
697701 25 µg ¥19,740
697702 100 µg ¥49,770
Description

Tuberous sclerosis 2 (TSC2) forms the tuberous sclerosis complex with TSC1 and functions as a crucial negative regulator of the Rheb/mTOR pathway. mTOR belongs to the PI3K-related kinase family and regulates protein translation, cell proliferation, and cell differentiation. Activation of mTOR requires binding to the active small GTPase Ras homolog enriched in brain (Rheb). TSC2 acts as a GTPase activating protein (GAP) and inhibits mTORC1 through converting active GTP-bound Rheb to inactive GDP-bound Rheb. Phosphorylation of TSC2 by AKT leads to inactivation of the tuberous sclerosis complex and in turn results in the activation of the Rheb/mTOR pathway. Mutations in the TSC2 gene lead to hyperactivation of the Rheb/mTOR pathway and are associated with the autosomal dominant disorder tuberous sclerosis and pulmonary lymphangioleiomyomatosis (LAM).

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human TSC2 recombinant protein (1508-1807 amino acid) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
KO/KD-WB - Verified 
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone does not work for IF application (in house tested).

Antibody specificity was confirmed by knock-down of TSC2 using siRNA (in-house tested). 

RRID
AB_2687204 (BioLegend Cat. No. 697701)
AB_2687188 (BioLegend Cat. No. 697702)

Antigen Details

Structure
1807 amino acids with a predicted molecular weight of 200 kD. Contains a Rap-GAP domain responsible for converting GTP-bound Rheb to its GDP-bound form.
Distribution

Cytoplasm. Membrane.

Function
TSC2 is a GTPase activating protein (GAP) and acts as a negative regulator of the Rheb/mTOR pathway.
Interaction
Interacts with TSC1, HERC1, HSPA1, HSPA8, DAPK1, FBXW5, NAA10, and RRAGA.
Biology Area
Cell Biology, Signal Transduction
Antigen References

1. Hobbs RM, et al. 2015. EMBO Rep. 16:467.
2. Demetriades C, et al. 2014. Cell. 156:786.
3. Tripathi DN, et al. 2013. Proc. Natl. Acad. Sci. USA. 110:E2950.
4. Han JM, et al. 2011. FEBS Lett. 585:973.
5. Huang J, et al. 2008. Biochem J. 412:179.
6. Li Y, et al. 2004. Trends Biochem Sci. 29:32.

Gene ID
7249 View all products for this Gene ID
UniProt
View information about TSC2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 0    Revision Date: 05/15/2017

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