Purified anti-Shiga toxin Stx2A Antibody

Pricing & Availability
Clone
1C6A6G9 (See other available formats)
Other Names
SLT-2 A subunit, SLT-2a, SLT-IIa, Verocytotoxin 2 subunit A, Verotoxin 2 subunit A, rRNA N-glycosidase 2, stxA2, stx2A
Isotype
Mouse IgG1, κ
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Product Citations
publications
1C6A6G9_Pure_ShigaToxin_Antibody_112117
Total protein (15 µg) from bacteria transformed with plasmid containing STX2A without IPTG induction (lane 1) and with IPTG induction ( lane 2) was resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:500 diluted (1 µg/mL) purified anti-Shiga toxin Stx2A antibody (clone 1C6A6G9) (upper). Proteins were visualized by chemiluminescence detection using 1:3000 diluted HRP Goat anti-mouse IgG Antibody (Cat. No. 405306). Ponceau S staining (lower) was used as loading control. M: protein marker.
  • 1C6A6G9_Pure_ShigaToxin_Antibody_112117
    Total protein (15 µg) from bacteria transformed with plasmid containing STX2A without IPTG induction (lane 1) and with IPTG induction ( lane 2) was resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:500 diluted (1 µg/mL) purified anti-Shiga toxin Stx2A antibody (clone 1C6A6G9) (upper). Proteins were visualized by chemiluminescence detection using 1:3000 diluted HRP Goat anti-mouse IgG Antibody (Cat. No. 405306). Ponceau S staining (lower) was used as loading control. M: protein marker.
Cat # Size Price Save
665903 25 µg ¥23,000
665904 100 µg ¥55,000
Description

The A subunit is responsible for inhibiting protein synthesis through the catalytic inactivation of 60S ribosomal subunits. After endocytosis, the A subnit is cleaved by furin in two fragments: A1, the catalytically active fragment, and A2, the fragment that is essential for holotoxin assembly with the B subunits.

Product Details
Technical data sheet

Product Details

Reactivity
Shiga toxin
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Peptide
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1:500-1:2500 diluted (0.2 - 1.0 µg/mL). It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone 1C6A6G9 recognizes epitope 198-212 (REFRQALSETAPVYT).

RRID
AB_2721751 (BioLegend Cat. No. 665903)
AB_2721750 (BioLegend Cat. No. 665904)

Antigen Details

Structure
A is a 35 kD subunit of Shiga toxin.
Biology Area
Bacterial proteins and Toxins, Cell Biology
Gene ID
1262249 View all products for this Gene ID
UniProt
View information about Stx2A on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 0    Revision Date: 11/27/2017

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