Purified anti-mouse F4/80 Antibody

Pricing & Availability
Clone
W20065D (See other available formats)
Regulatory Status
RUO
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W20065D_PURE_F480_Antibody_051022
Thioglycolate-elicited Balb/c mouse peritoneal macrophages were stained with anti-mouse CD11b APC and purified anti-mouse F4/80 (clone W20065D) (left) or purified rat IgG2b, ĸ isotype control (right), followed by PE anti-rat IgG.
  • W20065D_PURE_F480_Antibody_051022
    Thioglycolate-elicited Balb/c mouse peritoneal macrophages were stained with anti-mouse CD11b APC and purified anti-mouse F4/80 (clone W20065D) (left) or purified rat IgG2b, ĸ isotype control (right), followed by PE anti-rat IgG.
Cat # Size Price Save
111702 100 µg ¥25,410
Description

F4/80 is a 160 kD glycoprotein. It is characterized as a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80, also known as EMR1 or Ly71, has been widely used as a murine macrophage marker, which is expressed on the majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen (but not on the macrophages located in T cell areas of the spleen, lymph node and Peyer's patch), Kuffer cells, Langerhans cells, and bone marrow stromal cells. F4/80 has also been shown on a subset of dendritic cells. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for induction of CD8+ T cells-mediated peripheral tolerance.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2924451 (BioLegend Cat. No. 111702)

Antigen Details

Structure
EGF-TM7 family member, 160 kD glycoprotein
Distribution

Majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen, Kuffer cells, Langerhans cells, bone marrow stromal cells, and a subset of dendritic cells

Function
Induction of immunological tolerance
Cell Type
Macrophages, Monocytes
Biology Area
Immunology, Innate Immunity
Antigen References
  1. Austy JM and Gordon S. 1981. Eur. J. Immunol. 11:805.
  2. Hume DA, et al. 1983. J. Exp. Med. 158:1522.
  3. Ruedl C, et al. 1996. Eur. J. Immunol. 26:1801.
  4. McKnight AJ, et al. 1996. J. Biol. Chem. 271:486.
  5. Lin HH, et al. 2005. J. Exp. Med. 201:1615.
Gene ID
13733 View all products for this Gene ID
UniProt
View information about F4/80 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 05/10/2022

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  • Purified anti-mouse F4/80

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