Loading...

Purified anti-mouse CD326 (Ep-CAM) Antibody

Pricing & Availability
Clone
G8.8 (See other available formats)
Regulatory Status
RUO
Other Names
CD326, EGP40, MIC18, TROP1, KSA
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
G8dot8_Pure_060308
TE-71 cell line stained with G8.8 purified, followed by anti-rat IgG PE
  • G8dot8_Pure_060308
    TE-71 cell line stained with G8.8 purified, followed by anti-rat IgG PE
Compare all formats
Cat # Size Price Save
118201 50 µg ¥12,640
118202 500 µg ¥50,200
Description

EpCAM (CD326) mediates calcium-independent homophilic cell to cell adhesion. It may also function as a growth factor receptor. It is thought to be involved in maintaining cells in position during proliferation. Expression of EpCAM seems to correlate inversely with the level of E-cadherin (CD324). EpCAM is considered important in tumor biology.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
TE-71 thymic epithelial cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F, ICC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications for clone G8.8 (for the relevant formats) include: immunohistochemistry of frozen sections: acetone fixed1, with or without OCT embedding2,4, and spatial biology (IBEX)13,14.

Application References

(PubMed link indicates BioLegend citation)
  1. Farr A, et al. 1991. J. Histochem. Cytochem. 39:645. (FC, IHC)
  2. Dooley J, et al. 2005. J. Immunol. 175:4331. (FC, IHC)
  3. Hinterberger M, et. al. 2010. Nat. Immunol. 11:512. (FC) PubMed
  4. Gracz AD, et al. 2010. Am J. Physiol Gastrointest Liver Physiol. 298:590. (IHC) PubMed
  5. Nudel I, et al. 2011. J. Immunol. 186:891. PubMed
  6. Morimoto H, et al. 2012. Biol Reprod. 86:148. PubMed
  7. Ishii K, et al. 2012. Development. 139:1734. PubMed
  8. Takehashi M, et al. 2012. Biol Reprod. 86:178. PubMed
  9. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  10. Taguchi K, et al. 2014. Mol Cell Biol. 34:900. PubMed
  11. Hirokawa Y, et al. 2014. Am J Physiol Gastrointerest Liver Physiol. 306:547. PubMed
  12. Ding X, et al. 2015. Cancer Res. 75:330. PubMed
  13. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  14. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Vercauteren Drubbel A, et al. 2021. Cell Stem Cell. . PubMed
  2. Morimoto H, et al. 2012. Biol Reprod. 86:148. PubMed
  3. Thorsen AS, et al. 2020. Dis Model Mech. 14:dmm046706. PubMed
  4. Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
  5. Huang L, et al. 2020. JCI Insight. 5:00. PubMed
  6. Nudel I, et al. 2011. J Immunol. 186:891. PubMed
  7. Rieck M, et al. 2017. Eur J Immunol. 47:677. PubMed
  8. Casey AE, et al. 2018. J Cell Biol. 217:2951. PubMed
  9. Hirokawa Y, et al. 2014. Am J Physiol Gastrointest Liver Physiol. 306:547. PubMed
  10. Karabacak NM, et al. 2022. Cancer Res. 82:1084. PubMed
  11. Aparicio-Domingo P, et al. 2015. J Exp Med. 101084/jm20150318. PubMed
  12. Foster DS, et al. 2020. Nat Commun. 3.278472222. PubMed
  13. Ishii K, et al. 2012. Development. 139:1734. PubMed
  14. Hilligan KL, et al. 2020. Nat Commun. 11:5637. PubMed
  15. Lim PJ, et al. 2019. Nat Metab. 0.402083333. PubMed
  16. Liu H, et al. 2020. J Immunol. 205:1207. PubMed
  17. Vu LT, et al. 2019. J Extracell Vesicles. 8:1599680. PubMed
  18. Fuhrmann L, et al. 2021. Int J Mol Sci. 22:. PubMed
  19. E-Lacerda RR, et al. 2020. Clin Exp Allergy. 256:50. PubMed
  20. Corcos N, et al. 2021. Frontiers in Immunology. 12:616215. PubMed
  21. Taguchi K, et al. 2014. Mol Cell Biol. 34:900. PubMed
  22. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  23. Daley D, et al. 2017. Nat Med. 23:556. PubMed
  24. Sakamoto K, et al. 2021. Immunity. 54:2321. PubMed
  25. Billings SE, et al. 2021. Development. 148: . PubMed
  26. Thorsen AS, et al. 2020. Dis Model Mech. . PubMed
  27. Takehashi M, et al. 2012. Biol Reprod. 86:178. PubMed
  28. Tomchaney M, et al. 2021. BMC Pulm Med. 21:275. PubMed
  29. Link CWM, et al. 2020. Front Immunol. 11:596772. PubMed
  30. Yamamoto K, et al. 2022. STAR Protoc. 3:101155. PubMed
  31. Yamamoto K, et al. 2021. iScience. 24:103064. PubMed
  32. Molina LM, et al. 2021. Cell Reports. 36(1):109310. PubMed
  33. Blaskovic S, et al. 2020. Am J Physiol Lung Cell Mol Physiol. L606:318. PubMed
  34. Magaletta ME, et al. 2022. Nat Commun. 13:457. PubMed
  35. Burger ML, et al. 2021. Cell. 184:4996. PubMed
  36. Iyer N, et al. 2020. PLoS Pathog. 16:e1008360. PubMed
  37. Van Winkle JA, et al. 2020. J Virol. 94:. PubMed
  38. Zhou Y, et al. 2020. Am J Pathol. 190:1414. PubMed
  39. Wu W, et al. 2017. Sci Rep. 7:44481. PubMed
RRID
AB_1089026 (BioLegend Cat. No. 118201)
AB_1089027 (BioLegend Cat. No. 118202)

Antigen Details

Structure
40 kD single-pass type 1 glycoprotein. 293 amino acids, with a 21 aa signal peptide, a 246 aa extracellular domain, a 21 aa transmembrane domain, and a 26 aa cytoplasmic domain. The extracellular domain contains two epidermal growth factor-like repeats.
Distribution

Expressed on majority of epithelial cell membranes with the exception of adult squamous cells of the skin and a few specific epithelial cell types.

Function
Mediates calcium-independent homophilic cell-cell adhesion.
Interaction
CD326 displays hemophilic binding.
Ligand/Receptor
CD305 (LAIR-1), CD306 (LAIR-2), and Ep-CAM.
Cell Type
Embryonic Stem Cells, Epithelial cells
Biology Area
Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Borkowski TA, et al. 1996. Eur. J. Immunol. 26:110.
2. Bergsagel PL, et al. 1992. J. Immunol. 148:590.

Gene ID
17075 View all products for this Gene ID
UniProt
View information about CD326 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account