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- Clone
- W19132B (See other available formats)
- Regulatory Status
- RUO
- Other Names
- SignalingLymphocyte Activation Molecule (SLAM), IPO-3
- Isotype
- Rat IgG2a, λ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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C57BL/6 mouse splenocytes were stained with anti-mouse CD19 APC and purified anti-mouse CD150 (SLAM) (clone W19132B) (left) or rat IgG2a, κ isotype control (right), followed by anti-rat IgG PE.
CD150 is a 75-95 kD member of the immunoglobulin superfamily, also known as SLAM (signaling lymphocyte activation molecule) or IPO-3. CD150, a single chain type I transmembrane molecule, is expressed on thymocytes, T cell subsets, B cells, dendritic cells, and endothelial cells. The expression is upregulated upon activation. CD150 expression has been shown to be maintained on Th1 but not Th2 clones. T regulatory cells express a relatively high level of CD150. Antibodies against CD150 have been shown to augment IFN-γ production by Th1 cells, especially when co-stimulated through the TCR. CD150 associates with the src homology 2-domain-containing protein tyrosine phosphatase SHP-2, and this association is thought to be involved in signal transduction. In combination with CD48, CD150 is a useful marker for hematopoietic stem cell studies.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- RBL1 transfectant (1-287a.a)
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
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AB_2894667 (BioLegend Cat. No. 162602)
Antigen Details
- Structure
- Ig superfamily, 75-95 kD
- Distribution
-
Thymocytes, T cell subset, B lymphocytes, dendritic cells, endothelial cells
- Function
- B cell and dendritic cell costimulation
- Ligand/Receptor
- CD150
- Antigen References
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1. Punnonen J, et al. 1997. J. Exp. Med. 185:993.
2. Gordiienko I, et al. 2019. Clin Immunol. 204:14-22
3. Kiel M, et al. 2005. Cell. 121(7):1109-21 - Gene ID
- 27218 View all products for this Gene ID
- UniProt
- View information about CD150 on UniProt.org
Related FAQs
Other Formats
View All CD150 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-mouse CD150 (SLAM) Antibody | W19132B | FC |
| APC anti-mouse CD150 (SLAM) | W19132B | FC |
| PE anti-mouse CD150 (SLAM) | W19132B | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse CD150 (SLAM) Antibody
C57BL/6 mouse splenocytes were stained with anti-mouse CD19 ... -
APC anti-mouse CD150 (SLAM)
C57BL/6 mouse splenocytes were stained with anti-mouse CD19 ... -
PE anti-mouse CD150 (SLAM)
C57BL/6 mouse bone marrow cells were stained with anti-mouse...
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