Purified anti-mouse CD107a (LAMP-1) Antibody

Pricing & Availability
Clone
1D4B (See other available formats)
Regulatory Status
RUO
Other Names
Lysosome-associated membrane glycoprotein 1, lysosomal membrane glycoprotein 1, CD107 antigen-like family member A, lysosome-associated membrane protein 1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A_1D4B_Purified_083107
Thioglycollate-elicited BALB/c mouse peritoneal macrophages stained with purified 1D4B, followed by anti-rat IgG FITC
  • A_1D4B_Purified_083107
    Thioglycollate-elicited BALB/c mouse peritoneal macrophages stained with purified 1D4B, followed by anti-rat IgG FITC
  • B_1D4B_PURE_CD107a_LAMP1_WB_022708
    Western blot of purified anti-LAMP-1 antibody (clone 1D4B). Lane 1: Molecular weight marker; Lane 2: 20 µg of Hela cell lysate; Lane 3: 20 µg of NIH3T3 cell lysate; Lane 4: 20 µg of human brain lysate; Lane 5: 20 µg of mouse brain lysates; Lane 6: 10 ng human recombinant LAMP-1. The blot was incubated with 1 µg/ml of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-rat IgG (Cat. No. 405405). Anti-β-actin antibody was used as the loading control (Cat. No. 643807). Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Save
121601 50 µg ¥11,300
121602 200 µg ¥25,900
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
This monoclonal antibody was raised against NIH/3T3 mouse embryonic fibroblast tissue culture cell membranes. It has been mapped to the N-terminus of LAMP-1.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
WB - Verified
ICC, IP - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Zhou D, et al. 2018. Oncotarget. 9:5184. PubMed
  2. Wong AYW, et al. 2018. Front Immunol. 9:210. PubMed
  3. Reichenbach N, et al. 2018. J Exp Med. 215:1649. PubMed
  4. Reichenbach N, et al. 2019. EMBO Mol Med. 11:. PubMed
  5. Bussi C, et al. 2018. J Cell Sci. 131. PubMed
  6. Morioka S, et al. 2018. J Cell Sci. 131. PubMed
  7. Stacey MA, et al. 2017. J Clin Invest. 127:1463. PubMed
  8. Stelekati E, et al. 2018. Cell Rep. 2.445833333. PubMed
  9. Miyatake Y, et al. 2018. J Immunol. 201:3051. PubMed
  10. Tan CL, et al. 2018. Immunohorizons. 0.248611111. PubMed
  11. Wagner AK, et al. 2017. Nat Commun. 8:15627. PubMed
  12. Tan L, et al. 2017. Int J Nanomedicine. 12:3095. PubMed
  13. Daley D, et al. 2017. Nat Med. 23:556. PubMed
  14. Takemasu S, et al. 2019. J Biochem. 166:175. PubMed
  15. Steptoe R, et al. 2007. J Immunol . 178:2094. PubMed
  16. D'Agostino M, et al. 2011. J Cell Sci. 124:3545. PubMed
  17. Warburg G, et al. 2014. J Cell Biol. 204:423. PubMed
  18. Hasegawa J, et al. 2016. EMBO J. 35: 1853 - 1867. PubMed
  19. Morisaki Y, et al. 2016. Sci Rep. 6:27354. PubMed
  20. Stykel MG, et al. 2021. Cell Reports. 35(6):109099. PubMed
RRID
AB_572020 (BioLegend Cat. No. 121601)
AB_572021 (BioLegend Cat. No. 121602)

Antigen Details

Structure
LAMP-1 is a 406 amino acid protein with a molecular mass of 44 kD.
Gene ID
16783 View all products for this Gene ID
UniProt
View information about CD107a on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 02/27/2018

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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