- 11F4A09 (See other available formats)
- Other Names
- Interferon regulatory factor 5 (IRF-5)
- Mouse IgG2b, κ
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- Product Citations
IRF-5 is a transcription factor belonging to the interferon regulatory factor family. It controls induction of type I IFN genes as well as inflammatory cytokines and chemokines through TLRs and the MyD88-TRAF6 pathway. Several genetic variations of IRF-5 have been reported as a risk factor of Systemic lupus erythematosus (SLE), which is caused by excess IFNα induction.Product Details
- Antibody Type
- Host Species
- Partial human IRF-5 recombinant protein (176-240 aa).
- This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.05% sodium azide.
- 0.5 mg/ml
- Storage & Handling
- Upon receipt, store undiluted between 2°C and 8°C.
WB - Quality tested
IP - Validated
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 1.0 µg per ml. For immunoprecipitation, the suggested use of this reagent is 2-10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Internal testing suggests this clone is not suitable for intracellular flow cytometry.
AB_2561469 (BioLegend Cat. No. 652601)
AB_2561470 (BioLegend Cat. No. 652602)
- 498 aa with predicted molecular weight of 56 kD. The protein contains a DNA binding domain (IRF tryptophan pentad repeat) and a poly-Glutamate region.
Shuttles between the nucleus and the cytoplasm.
- The transcription factor IRF-5 participates in the virus-mediated activation of interferon and regulates gene expression induced by cell growth, differentiation, and apoptosis.
- IKKα phosphorylates IRF-5 and induces formation of IRF-5 dimers. TRAF6 mediates K63-linked ubiquitination of IRF-5, required for nuclear translocation of IRF-5 and regulation of target genes. IRF-5 assemblies with CBP/p300 to initiate transcription.
- Cell Type
- Dendritic cells
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Signal Transduction, Transcription Factors
- Antigen References
1. Balkhi MY, et al. 2010. Cell. Signal. 1:117.
2. Balkhi MY, et al. 2008. Mol. Cell. Biol. 28:7296.
3. Salloum R and Niewold TB. 2011. Transl. Res. 157:326.
4. Barnes BJ, et al. 2003. J. Biol. Chem. 278:16630.
5. Rullo OJ, et al. 2010. Ann. Rheum. Dis. 69:611.
- Gene ID
- 3663 View all products for this Gene ID
- View information about IRF5 on UniProt.org
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