- A15076D (See other available formats)
- Regulatory Status
- Other Names
- IL-12 subunit p40, IL-23 subunit p40, Cytotoxic lymphocyte maturation factor, CLMF2, Natural killer cell stimulatory factor 40-KD subunit, NKSF2
- Mouse IgG2b, κ
- Ave. Rating
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- Product Citations
IL-12 and IL-23 share the p40 subunit, which heterodimerizes respectively with IL-12 p35 or IL-23 p19 subunits to form IL-12 or IL-23. IL-12 p40 exists as a monomer and as a homodimer (IL-12 p80). IL-12 acts as a growth factor for activated human T and NK cells, enhances the lytic activity of human NK cells, and stimulates the production of IFN-γ by resting human PBMC. IL-12R is formed by two chains, IL-12Rβ1 and IL-12Rβ2. IL-12Rβ1 is associated with the Janus kinase (Jak) Tyk2 and binds IL-12 p40; IL-12Rβ2 is associated with Jak2 and binds either the heterodimer or the p35 chain. Signaling through the IL-12 receptor complex induces phosphorylation, dimerization, and nuclear translocation of several signal transducers and activators of transcription (STAT) family members (STAT1, 3, 4, 5), but most of the biological responses to IL-12 have been attributed to STAT4. IL-12 has been shown to elicit anti-tumor activity in mice and humans. It is believed that the antitumor effects of IL-12 are mediated, at least in part, by indirect mechanisms. Induction of IFN-γ results in the upregulation of class I and class II MHC molecules, adhesion molecules (ICAM-1), nitric oxide production by antigen presenting cells (APC), and the production of additional cytokines, CXCL9 and 10, which in turn mediate angiostatic effects.Product Details
- Antibody Type
- Host Species
- Recombinant human IL-12.
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
WB - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Product Citations
AB_2632765 (BioLegend Cat. No. 692502)
- 328 amino acids with a predicted molecular weight of 40 kD.
- p40 homodimer functions as a proinflammatory protein that enhances leukocyte accumulation in the skin, blunt Th1 immunity to Plasmodium berghei, and provides protective immunity toward mycobacterial infection. In addition, p40 homodimer induces macrophage chemotaxis independent of IL-12. Also, IL-12 p40 homodimer (p402) induces the expression of inducible nitric oxide synthase (iNOS) in microglia.
- Heterodimer with IL12A and IL23A.
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Signal Transduction
- Molecular Family
- Antigen References
1. Schoenhaut DS, et al. 1992. J. Immunol. 148:3433.
2. Manetti R, et al. 1994. J. Exp. Med. 179:1273.
3. Ireland D, et al. 2005. Viral Immunol. 18:397.
4. Moreno SE, et al. 2006. J. Immunol. 177:3218.
5. Lyakh L, et al. 2008. Immunol. Rev. 226:112.
6. Theiner G, et al. 2008. Mol. Immunol. 45:244.
7. Zhu S, et al. 2010. J. Immunol. 184:2348.
- Gene ID
- 3593 View all products for this Gene ID
- View information about IL-12 p40 on UniProt.org
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