- Poly5360 (See other available formats)
- Regulatory Status
- Other Names
- Monocyte chemoattractant protein-1 (MCP1), Monocyte chemoattractant and activating factor (MCAF), JE, Small inducible cytokine A2 (SCYA2), HC-11 (HC11), P6, Smooth muscle cell chemotactic factor (SMC-CF, SMCCF), CCL2, MCP-1, MCP1,CCL-2, MCP-1/CCL2
- Goat Ig
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- Product Citations
MCP-1, also known as CCL2, is a member of the CC chemokine family. It is widely expressed in endothelial cells, smooth muscle cells and monocytes in response to several atherogenic stimulants such as CD40 ligand, platelet derived growth factor (PDGF), interleukin-1β (IL-1β) and oxidized low density lipoprotein. In addition, MCP-1 has been implicated in monocytic infiltration of tissues during several inflammatory diseases, and has been implicated in macrophage-mediated tumor growth suppression in mice. MCP-1 has been shown to have direct effects on tumor cells in an autocrine and paracrine fashion in multiple cancers, including breast, lung, cervical, ovarian, and prostatic. In addition, MCP-1 plays a key role in the regulation of MMPs during transmigration.Product Details
- Antibody Type
- Host Species
- Recombinant protein
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
ELISA Capture - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by ELISA assay. For ELISA Capture applications, a concentration range of 0.5 - 4.0 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of MCP-1 recombinant protein ranging from 1000 to 15.6 pg/mL is recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
The purified Poly5360 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated Poly5360 antibody (Cat. No. 536004) as the detection antibody and Recombinant Human MCP-1 (CCL2) (ELISA Std.) (Cat. No. 565001).
AB_2734496 (BioLegend Cat. No. 536002)
- CC chemokine, dimer, 13.8 kD (Mammalian)
Monocytes, vascular endothelial cells, smooth muscle, glomerular mesangial cells
- Upregulated by IL-1, TNF-α, PDGF, TGF-β
- Monocytes, basophils, T cells, NK cells, immature dendritic cells
- CCR2, CCR5, CCR10, D6, possibly L-CCR
- Cell Type
- Monocytes, Endothelial cells
- Biology Area
- Cell Biology, Neuroinflammation, Neuroscience
- Molecular Family
- Antigen References
1. Fitzgerald KA, et al. 2001. The Cytokine FactsBook; Academic Press, San Diego.
2. Bischoff SC, et al. 1992. J. Exp. Med. 175:1271.
3. Charo IF, et al. 1994. Proc. Natl. Acad. Sci. USA. 91:2752.
4. Jiang Y, et al. 1992. J. Immunol. 148:2423.
- Gene ID
- 6347 View all products for this Gene ID
- View information about MCP-1 on UniProt.org
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