Purified anti-human FGF-basic Antibody

Pricing & Availability
Clone
BHFB15 (See other available formats)
Regulatory Status
RUO
Other Names
Fibroblast Growth Factor-basic, bFGF, FGF-2, FGF2, Heparin-binding growth factor
Isotype
Mouse IgG1, κ
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Product Citations
publications
Cat # Size Price Save
514801 50 µg ¥18,000
514802 500 µg ¥48,000
Description

Fibroblast growth factor-basic (also known as FGF-b, FGF-2) is a heparin-binding growth factor, which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal, and endothelial cells. FGF-basic also exerts a potent angiogenic activity in vivo. FGF-basic has been isolated from neural, pituitary, adrenal cortex, and placental tissues. The BHFB15 antibody reacts with human fibroblast growth factor - basic (FGF-basic).

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human FGF-basic.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration of 2 µg/ml of the capture antibody was utilized to generate the example standard curve. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Capture: The purified BHFB15 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated JKFb-2 antibody as the detecting antibody.

RRID
AB_2561316 (BioLegend Cat. No. 514801)
AB_2561317 (BioLegend Cat. No. 514802)

Antigen Details

Structure
17.2 kD (Mammalian)
Distribution

Brain, retina, pituitary, kidney, placenta, testis, corpus luteum, adrenal glands, monocytes, prostate, bone, liver, cartiliage, endothelial cells, and epithelial cells.

Function
Released after tissue injuries and during the inflammatory process.
Interaction
Fibroblasts, myobalasts, osteoblasts, neuronal cells, endothelial cells, kerintocytes, chrondrocytes, astrocytes, oligodendrocytes, and the smooth muscle.
Ligand/Receptor
FGFR1-1 (flg); FGFR-2 (bek, K-sam); FGFR-4 (flig-2); low affinity coreceptor heparin sulfate, and heparin sulfate proteoglycans required for full activity.
Cell Type
Monocytes, Epithelial cells, Endothelial cells
Biology Area
Angiogenesis, Cell Biology, Immunology, Neuroscience, Synaptic Biology
Molecular Family
Adhesion Molecules, Cytokines/Chemokines, Growth Factors
Antigen References

1. Fitzgerald KA, et al. 2013. The Cytokine Factsbook. Acadmic Press. San Diego.
2. Klagsbrun M, 1992. Semin. Cancer Biol. 3:81.
3. Goldfarb M. 1990. Cell Growth Factor. 1:439.
4. Sensenbrenner M. 1993. Prog. Neurobiol. 6:683.

Gene ID
2247 View all products for this Gene ID
UniProt
View information about FGF-basic on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 01/07/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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