Purified anti-human CD19 Antibody

Pricing & Availability
SJ25C1 (See other available formats)
Regulatory Status
Other Names
CD19 Molecule, CD19 Antigen, Differentiation Antigen CD19, B-Lymphocytes Surface Antigen B4, T-cell Surface Antigen Leu-12, CVID3, B4, B-lymphocyte Antigen CD19
Mouse IgG1, κ
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Product Citations
Human peripheral blood lymphocytes were stained with purified CD19 (clone SJ25C1, closed histogram) or mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG PE.
  • A_SJ25C1_PURE_CD19_FC_081214.jpg
    Human peripheral blood lymphocytes were stained with purified CD19 (clone SJ25C1, closed histogram) or mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG PE.
  • B_SJ25C1_PURE_CD19_IHCF_032318
    Frozen human tonsil section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of purified anti-human CD19 (clone SJ25C1) overnight at 4°C, followed by 2.5 µg/ml of Alexa Fluor® 594 anti-mouse IgG (clone Poly4053) (red) for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured by 10X objective.
Compare all formats
Cat # Size Price Save
363001 25 µg ¥11,880
363002 100 µg ¥24,640

CD19 is a 95 kD type I transmembrane glycoprotein also known as B4. It is a member of the immunoglobulin superfamily expressed on B cells (from pro-B to blastoid B cells, absent on plasma cells) and follicular dendritic cells. CD19 is involved in B cell development, activation, and differentiation. CD19 forms a complex with CD21 (CR2) and CD81 (TAPA-1), and functions as a BCR co-receptor.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Antibody Type
Host Species
NALM1 + NALM16 cells
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.

FC - Quality tested
IHC-F - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 5.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone SJ25C1 partially blocks anti-human CD19 clones HIB19 and 4G7 staining based on in-house testing

Product Citations
  1. Zhao J, et al. 2017. Sci Immunol. 2:eaan5400. PubMed
  2. Stensland ZC, et al. 2022. iScience. 25:103626. PubMed
AB_2563988 (BioLegend Cat. No. 363001)
AB_2563989 (BioLegend Cat. No. 363002)

Antigen Details

Ig superfamily, type I transmembrane glycoprotein, 95 kD.

B lineage (except plasma cells), follicular dendritic cells.

B cell activation and differentiation.
Forms a complex with CD21, CD81 and CD225 in the membrane of mature B cells and interacts with VAV and LYN. It also interacts with GRAB2 and SOS when phosphorylated on Tyr-348 and/or Tyr-378 and with PLCG2 when phosphorylated on Tyr-409.
Forms complex with CD21 (CR2) and CD81 (TAPA-1), BCR co-receptor.
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Tedder T, et al. 1994. Immunol. Today 15:437.
2. Bradbury L, et al. 1993. J. Immunol. 151:2915.

Gene ID
930 View all products for this Gene ID
View information about CD19 on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 03/07/2022

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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