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- Clone
- W6D3 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- HCDM listed
- Other Names
- Lewis X, 3-FAL, 3-FL, LNFP III, Lex , SSEA-1, X-hapten
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Human PBMCs (blue) and RBC-lysed human whole blood cells (red) stained with 164Dy-anti-CD15 (W6D3). Data provided by DVS Sciences.
CD15 is 3-fucosyl-N-acetyllactosamine (3-FAL) also known as Lewis X, 3-FAL, X-hapten, and SSEA-1. CD15 is expressed on granulocytes and monocytes. It has also been shown to be expressed on Langerhans cells. CD15 has been implicated in adhesion as well as chemotaxis, phagocytosis, and bactericidal activity.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- WERI-RB-1 retinoblastoma cell line
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1.0 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
CyTOF® - Verified - Recommended Usage
-
This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.
- Additional Product Notes
-
Maxpar® is a registered trademark of Standard BioTools Inc.
- RRID
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AB_2562831 (BioLegend Cat. No. 323035)
Antigen Details
- Structure
- Poly-N-acetyllactosamine
- Distribution
-
Neutrophils, eosinophils, monocytes
- Function
- Adhesion
- Cell Type
- Embryonic Stem Cells, Eosinophils, Neutrophils
- Biology Area
- Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Molecular Family
- CD Molecules
- Antigen References
-
1. Stocks SC, et al. 1990. Biochem. J. 268:275.
- Gene ID
- 2526 View all products for this Gene ID
- UniProt
- View information about CD15 on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
- Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?
-
We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.
- Can I use Maxpar® Ready format clones for flow cytometry staining?
-
We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.
- I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.
-
We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/
- Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?
-
The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.
Other Formats
View All CD15 Reagents Request Custom ConjugationCustomers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Brilliant Violet 785™ anti-human CD15 (SSEA-1)
Human peripheral blood granulocytes were stained with CD15 (... -
Purified anti-human CD15 (SSEA-1)
Human peripheral blood granulocytes stained with purified W6... -
FITC anti-human CD15 (SSEA-1)
Human peripheral blood granulocytes stained with W6D3 FITC -
PE anti-human CD15 (SSEA-1)
Human peripheral blood granulocytes stained with W6D3 PE -
APC anti-human CD15 (SSEA-1)
Human peripheral blood granulocytes stained with W6D3 APC -
Alexa Fluor® 488 anti-human CD15 (SSEA-1)
Human peripheral blood granulocytes stained with W6D3 Alexa ... -
Alexa Fluor® 647 anti-human CD15 (SSEA-1)
Human peripheral blood granulocytes stained with W6D3 Alexa ... -
PE/Cyanine5 anti-human CD15 (SSEA-1)
Human peripheral blood granulocytes stained with W6D3 PE/Cya... -
PerCP anti-human CD15 (SSEA-1)
Human peripheral blood granulocytes stained with W6D3 PerCP -
PerCP/Cyanine5.5 anti-human CD15 (SSEA-1)
Human peripheral blood granulocytes were stained with CD15 (... -
Pacific Blue™ anti-human CD15 (SSEA-1)
Human peripheral blood granulocytes stained with W6D3 Pacifi... -
Alexa Fluor® 700 anti-human CD15 (SSEA-1)
Human peripheral blood granulocytes were stained with CD15 (... -
Brilliant Violet 510™ anti-human CD15 (SSEA-1)
Human peripheral blood granulocytes were stained with CD15 (... -
PE/Cyanine7 anti-human CD15 (SSEA-1)
Human peripheral blood granulocytes were stained with CD15 (... -
Brilliant Violet 605™ anti-human CD15 (SSEA-1)
Human peripheral blood granulocytes were stained with CD15 (... -
Brilliant Violet 650™ anti-human CD15 (SSEA-1)
Human peripheral blood granulocytes were stained with CD15 (... -
Purified anti-human CD15 (SSEA-1) (Maxpar® Ready)
Human PBMCs (blue) and RBC-lysed human whole blood cells (re... -
PE/Dazzle™ 594 anti-human CD15 (SSEA-1)
Human peripheral blood granulocytes were stained with CD15 P... -
Brilliant Violet 421™ anti-human CD15 (SSEA-1)
Human peripheral blood granulocytes were stained with CD15 (... -
APC/Fire™ 750 anti-human CD15 (SSEA-1)
Human peripheral blood granulocytes were stained with CD15 (... -
FITC anti-human CD15 (SSEA-1)
Typical results from human peripheral blood granulocytes sta... -
Pacific Blue™ anti-human CD15 (SSEA-1)
Typical results from human peripheral blood granulocytes sta... -
TotalSeq™-A0392 anti-human CD15 (SSEA-1)
-
Brilliant Violet 711™ anti-human CD15 (SSEA-1)
Human peripheral blood granulocytes were stained with CD15 (... -
APC/Cyanine7 anti-human CD15 (SSEA-1)
Human peripheral blood granulocytes were stained with CD15 (... -
TotalSeq™-B0392 anti-human CD15 (SSEA-1)
-
TotalSeq™-C0392 anti-human CD15 (SSEA-1)
-
PE anti-human CD15 (SSEA-1)
Typical results from human peripheral blood granulocytes sta... -
APC/Fire™ 810 anti-human CD15 (SSEA-1) Antibody
Human peripheral blood granulocytes were stained with anti-h... -
TotalSeq™-D0392 anti-human CD15 (SSEA-1)
-
GMP FITC anti-human CD15 (SSEA-1)
Typical results from human peripheral blood granulocytes sta... -
Spark YG™ 593 anti-human CD15 (SSEA-1)
Human peripheral blood granulocytes were stained with anti-h... -
GMP PE anti-human CD15 (SSEA-1)
Typical results from human peripheral blood granulocytes sta... -
GMP Pacific Blue™ anti-human CD15 (SSEA-1)
Typical results from human peripheral blood granulocytes sta... -
Spark Violet™ 500 anti-human CD15 (SSEA-1)
Human peripheral blood granulocytes were stained with anti-h...
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