Purified anti-human CD134 OX40 Maxpar Ready Antibody anti-CD134

Clone: Ber-ACT35 (ACT35) (See other available formats)
Regulatory Status: RUO
Other Names: ACT35 antigen, tumor necrosis factor receptor superfamily member 4 (TNFRSF4)
Isotype: Mouse IgG1, κ
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Product Citations: publications

Human PBMCs were incubated for 3 days in media alone (top) or with PHA (bottom) and then stained with 154Sm-anti-CD45 (HI30) and 158Gd-anti-CD134 (ACT35). CD45+CD14- cells are displayed in the analysis. Data provided by DVS Sciences.

Cat #	Size	Price	Save
350015	100 µg	¥32,340

Description

CD134, also known as OX40 and TNFRSF4, is a 50 kD type I transmembrane glycoprotein. It is a member of the TNF receptor family. OX40 is expressed on activated T lymphocytes including Th1, Th2, Th17, and Treg cells. The interaction of OX40 with OX40L results in B cell proliferation and antibody secretion, regulation of primary T cell expansion, and T cell survival. OX40 influences the size of the T cell memory pool and regulation of CD4⁺ T cell tolerance.

Product Details

Technical data sheet

Product Details

Verified Reactivity

Human

Reported Reactivity

Chimpanzee

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

HTLV 1-transformed HUT 102 cells

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.

Preparation

The antibody was purified by affinity chromatography.

Concentration

1.0 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

FC - Quality tested
CyTOF® - Verified

Recommended Usage

This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.

Application Notes

Additional reported applications (for the relevant formats) include: Western blotting¹, immunoprecipitation¹, immunohistochemical staining^2,3of paraffin embedded⁷ and frozen tissue sections, ELISA⁴, and functional assay⁵. The LEAF™ or Ultra-LEAF™ purified antibody is recommended for functional assays (contact our custom solutions team).

Additional Product Notes

Maxpar® is a registered trademark of Standard BioTools Inc.

Application References (PubMed link indicates BioLegend citation)

Latza U, et al. 1994. Eur. J. Immunol. 24:677. (WB, IP)
Durkop H, et al. 1995. Brit. J. Haematol. 91:927. (IHC)
Durkop H, et al. 1997. Brit. J. Haematol. 98:863. (IHC)
Willett B, et al. 2007. J. Virol. 81:9665. (ELISA)
Li M and Zhang Y. et al. 2005. Cell. Mol. Immunol. 2:467. (FA)
Gloviczki ML, et al. 2012. Clin. J. Am. Soc. Nephrol. 8:546. PubMed
Domingos PL, et al. 2012. An. Bras. Dermatol. 87:851. (IHC)

Product Citations

Gide TN, et al. 2019. Cancer Cell. 35:238. PubMed

RRID

AB_2563718 (BioLegend Cat. No. 350015)

Antigen Details

Structure: 50 kD, TNF receptor family
Distribution: Activated T cells
Function: Receptor for OX40 ligand, co-stimulatory signal for T cell proliferation and survival.
Ligand/Receptor: OX40L (CD252)
Cell Type: T cells, Tregs
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Costimulatory Molecules, Immunology
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: 1. Smith CA, et al. 1994. Cell. 76:959.
2. Chen AL, et al. 1999. Immunity. 11:689.
3. Croft M. 2010. Annu. Rev. Immunol. 28:57.
4. Withers DR, et al. 2009. J. Immunol. 183:5079.
5. Klinger M, et al. 2009. J. Immunol. 182:4581.
Gene ID: 7293 View all products for this Gene ID
UniProt: View information about CD134 on UniProt.org

Documentation

Reviews

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related Pages & Pathways

Pathways

Related FAQs

Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?

We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm^®. Please contact Fluidigm^® directly for additional data and further details.

http://techsupport.fluidigm.com/

Can I use Maxpar® Ready format clones for flow cytometry staining?

We have not tested the Maxpar^® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.

I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.

We only supply the antibody and not test that in house. Please contact Fluidigm^® directly for troubleshooting advice: http://techsupport.fluidigm.com/

Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?

The Maxpar^® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.

Other Formats

View All CD134 Reagents Request Custom Conjugation

Description	Clone	Applications
FITC anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	FC
Purified anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	FC,IHC-P,ELISA,IHC-F,WB
PE anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	FC
APC anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	FC
PerCP/Cyanine5.5 anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	FC
PE/Cyanine7 anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	FC
Brilliant Violet 421™ anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	FC
Purified anti-human CD134 (OX40) (Maxpar® Ready)	Ber-ACT35 (ACT35)	FC,CyTOF®
Alexa Fluor® 647 anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	FC
PE/Dazzle™ 594 anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	FC
APC/Cyanine7 anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	FC
Biotin anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	FC
Brilliant Violet 510™ anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	FC
Brilliant Violet 605™ anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	FC
APC/Fire™ 750 anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	FC
Brilliant Violet 711™ anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	FC
TotalSeq™-A0158 anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	PG
TotalSeq™-C0158 anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	PG
TotalSeq™-B0158 anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	PG
TotalSeq™-D0158 anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	PG
PE/Cyanine5 anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	FC
PE/Fire™ 810 anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	FC
PE anti-human CD134	Ber-ACT35 (ACT35)	FC
PE/Fire™ 744 anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	FC

Certificate of Analysis

Go To Top Version: 2 Revision Date: 07/22/2022

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.