Purified anti-human CD11b Maxpar Ready Antibody anti-CD11b

Clone: ICRF44 (See other available formats)
Regulatory Status: RUO
Workshop: IV M047
Other Names: Integrin αM chain, C3biR, CR3, Mac-1, Mo1, ITGAM
Isotype: Mouse IgG1, κ
Ave. Rating: Submit a Review
Product Citations: publications

ICRF44_Purified_CD11b_CyTOF_112213 — Human PBMCs stained with 144Nd-anti-CD11b (ICRF44). T lymphocytes (bottom) and monocytes (top) are displayed in the analysis. Data provided by DVS Sciences.

Cat #	Size	Price	Save
301337	100 µg	¥31,020

Description

CD11b is a 165-170 kD type I transmembrane glycoprotein also known as α_M integrin, Mac-1, CR3, and C3biR. CD11b non-covalently associates with integrin β₂ (CD18) and is expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b/CD18 is critical for the transendothelial migration of monocytes and neutrophils. It is also involved in granulocyte adhesion, phagocytosis, and neutrophil activation. CD11b/CD18 interacts with ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4, CD14, CD23, heparin, iC3b, fibrinogen, and factor X.

Product Details

Technical data sheet

Product Details

Verified Reactivity

Human, Cynomolgus, Rhesus

Reported Reactivity

African Green, Baboon, Chimpanzee, Common Marmoset, Pig

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.

Preparation

The antibody was purified by affinity chromatography.

Concentration

1.0 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

FC - Quality tested
CyTOF® - Verified

Recommended Usage

This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.

Application Notes

The ICRF44 antibody inhibits heterotypic adhesion of granulocytes in response to fMLP. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections, immunofluorescence microscopy⁵, stimulation of monocytes³, blocking of heterotypic PMN aggregation⁸, and blocking of granulocyte activation¹². This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue.

The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 301361 & 301362).

Additional Product Notes

Maxpar® is a registered trademark of Standard BioTools Inc.

Application References (PubMed link indicates BioLegend citation)

Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
Rezzonico R, et al. 2001. Blood 97:2932. (Stim)
Marsik C, et al. 2003. Shock 20:493. (FC)
David A, et al. 2003. J. Leukoc. Biol. 74:551. (IF)
Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
Thurlow LR, et al. 2010. Infect. Immun. 128:1128. (FC) PubMed
Jadhav S, et al. 2001. J. Immunol. 167:5986. (Block)
Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21. (FC)
Wen T, et al. 2014. J Immunol. 192:5481. (FC) PubMed
Sprong T, et al. 2003. Blood 102:3702. (Block)
Cash JL, et al. 2013. EMBO Rep. 14:999. (FC) PubMed
Larsson K, et al. 2015. PNAS. PubMed

Product Citations

Gañán-Gómez I, et al. 2022. Nat Med. . PubMed
Senosain MF, et al. 2021. Sci Rep. 11:14424. PubMed
Georg P, et al. 2022. Cell. 185:493. PubMed

RRID

AB_2562811 (BioLegend Cat. No. 301337)

Antigen Details

Structure: Integrin, type I transmembrane glycoprotein, associates with integrin β₂ (CD18), 165-170 kD
Distribution: Granulocytes, monocytes/macrophages, dendritic cells, NK cells, subset of T cells, subset of B cells
Function: Adhesion, phagocytosis, chemotaxis, neutrophil activation
Ligand/Receptor: ICAM-1(CD54), ICAM-2 (CD102), ICAM-4, CD14, CD23, heparin, iC3b, fibrinogen, factor X
Cell Type: B cells, Dendritic cells, Granulocytes, Macrophages, Monocytes, Neutrophils, NK cells, T cells, Tregs
Biology Area: Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Stewart M, et al. 1995. Curr Opin Cell Biol. 7:690.
Gene ID: 3684 View all products for this Gene ID
UniProt: View information about CD11b on UniProt.org

Documentation

Reviews

Review This Product

Related Protocols

Cell Surface Flow Cytometry Staining Protocol

Related Products

Description	Clone	Applications

Related Pages & Pathways

Pathways

Related FAQs

Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?

We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm^®. Please contact Fluidigm^® directly for additional data and further details.

http://techsupport.fluidigm.com/

Can I use Maxpar® Ready format clones for flow cytometry staining?

We have not tested the Maxpar^® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.

I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.

We only supply the antibody and not test that in house. Please contact Fluidigm^® directly for troubleshooting advice: http://techsupport.fluidigm.com/

Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?

The Maxpar^® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.

Other Formats

View All CD11b Reagents Request Custom Conjugation

Description	Clone	Applications
APC anti-human CD11b	ICRF44	FC
Biotin anti-human CD11b	ICRF44	FC
PE anti-human CD11b	ICRF44	FC
PE/Cyanine5 anti-human CD11b	ICRF44	FC
Purified anti-human CD11b	ICRF44	FC,CyTOF®,ICC,IHC
Pacific Blue™ anti-human CD11b	ICRF44	FC
Alexa Fluor® 488 anti-human CD11b	ICRF44	FC
Alexa Fluor® 647 anti-human CD11b	ICRF44	FC
PE/Cyanine7 anti-human CD11b	ICRF44	FC
PerCP/Cyanine5.5 anti-human CD11b	ICRF44	FC
Brilliant Violet 421™ anti-human CD11b	ICRF44	FC,ICC
Brilliant Violet 570™ anti-human CD11b	ICRF44	FC
FITC anti-human CD11b	ICRF44	FC
Brilliant Violet 605™ anti-human CD11b	ICRF44	FC
Brilliant Violet 510™ anti-human CD11b	ICRF44	FC,ICC
Brilliant Violet 650™ anti-human CD11b	ICRF44	FC
Purified anti-human CD11b (Maxpar® Ready)	ICRF44	FC,CyTOF®
Alexa Fluor® 594 anti-human CD11b	ICRF44	ICC,FC
APC/Cyanine7 anti-human CD11b	ICRF44	FC
Brilliant Violet 711™ anti-human CD11b	ICRF44	FC
Brilliant Violet 785™ anti-human CD11b	ICRF44	FC
PE/Dazzle™ 594 anti-human CD11b	ICRF44	FC
APC/Fire™ 750 anti-human CD11b	ICRF44	FC
APC anti-human CD11b	ICRF44	FC
PE anti-human CD11b	ICRF44	FC
TotalSeq™-A0161 anti-human CD11b	ICRF44	PG
Alexa Fluor® 700 anti-human CD11b	ICRF44	FC
PE/Cyanine7 anti-human CD11b	ICRF44	FC
TotalSeq™-B0161 anti-human CD11b	ICRF44	PG
TotalSeq™-C0161 anti-human CD11b	ICRF44	PG
PerCP/Cyanine5.5 anti-human CD11b	ICRF44	FC
Ultra-LEAF™ Purified anti-human CD11b	ICRF44	FC,CyTOF®,Block,ICC,IHC
TotalSeq™-D0161 anti-human CD11b	ICRF44	PG
GMP PE/Cyanine7 anti-human CD11b	ICRF44	FC
Pacific Blue™ anti-human CD11b	ICRF44	FC
GMP PE anti-human CD11b	ICRF44	FC
Spark UV™ 387 anti-human CD11b	ICRF44	FC
GMP PerCP/Cyanine5.5 anti-human CD11b	ICRF44	FC
FITC anti-human CD11b	ICRF44	FC
APC/Fire™ 750 anti-human CD11b	ICRF44	FC
GMP APC anti-human CD11b	ICRF44	FC

Certificate of Analysis

Go To Top Version: 6 Revision Date: 07/21/2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.