Purified anti-GFAP Antibody (Previously Covance catalog# PCK-591P)

Pricing & Availability
Clone
Poly28294 (See other available formats)
Regulatory Status
RUO
Other Names
Glial fibrillary acidic protein
Previously
Covance Catalog# PCK-591P
Isotype
Chicken IgY
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Product Citations
publications
Poly28294_Purified_GFAP_Antibody_1_111319
IHC staining of purified anti-GFAP antibody (clone Poly28294) on formalin-fixed paraffin-embedded human brain tissue. The tissue was incubated with a 1:5,000 dilution of the primary antibody for 20 minutes at room temperature. For detection, a biotinylated anti-chicken secondary was followed by the HRP labeling reagent and DAB from BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • Poly28294_Purified_GFAP_Antibody_1_111319
    IHC staining of purified anti-GFAP antibody (clone Poly28294) on formalin-fixed paraffin-embedded human brain tissue. The tissue was incubated with a 1:5,000 dilution of the primary antibody for 20 minutes at room temperature. For detection, a biotinylated anti-chicken secondary was followed by the HRP labeling reagent and DAB from BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • Poly28294_Purified_GFAP_Antibody_2_111319
    IHC staining of purified anti-GFAP antibody (clone Poly28294) on formalin-fixed paraffin-embedded mouse brain tissue. The tissue was incubated with a 1:5,000 dilution of the primary antibody for 20 minutes at room temperature. For detection, a biotinylated anti-chicken secondary was followed by the HRP labeling reagent and DAB from BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • Poly28294_Purified_GFAP_Antibody_3_111319
    IHC staining of purified anti-GFAP antibody (clone Poly28294) on formalin-fixed paraffin-embedded rat brain tissue. The tissue was incubated with a 1:5,000 dilution of the primary antibody for 20 minutes at room temperature. For detection, a biotinylated anti-chicken secondary was followed by the HRP labeling reagent and DAB from BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • Poly28294_Purified_GFAP_Antibody_4_111319
    Western blot of purified anti-GFAP antibody (clone Poly28294). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with a 1:10,000 dilution of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-chicken. Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Save
829401 100 µL ¥84,600
Description

Glial fibrillary acidic protein (GFAP) is the 10nm or intermediate filament protein found specifically in astrocytic cells in the central nervous system. Antibodies to GFAP are therefore widely used to identify astrocytic cells in situ and in tissue culture. This antibody was developed using bovine spinal cord GFAP purified from a Triton X-100 extract of myelin associated material and further purified by centrifugation and ion exchange chromatography in 6m urea on DEAE cellulose.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Polyclonal
Host Species
Chicken
Immunogen
Native GFAP purified from bovine spinal cord.
Formulation
Phosphate-buffered solution, containing 0.2% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

IHC-P - Quality tested
WB - Verified

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a dilution range of 1:1000 - 1:5000 is suggested. For Western blotting, the suggested use of this reagent is a 1:5000 - 1:10000 dilution. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Sosunov AA, et al. 2013. J. Neurosci. 33:7439-50. (IHC, WB) PubMed
Product Citations
  1. Li J, et al. 2019. Glia. 67:1571. PubMed
  2. Sosunov A, et al. 2013. J Neurosci. 33:7439-7450. PubMed
  3. Rao SB, et al. 2021. Biochimica et Biophysica Acta (BBA) - Biomembranes. 1863(8):183616. PubMed
RRID
AB_2564929 (BioLegend Cat. No. 829401)

Antigen Details

Structure
GFAP is a 432 amino acid protein with a molecular mass of ~50 kD.
Distribution

Tissue distribution: GFAP is expressed by numerous cell types of the central nervous system (CNS) including astrocytes, ependymal cells, and Bergmann glia cells (protoplasmic astrocyte). GFAP is expressed in cells lacking fibronectin.
Cellular distribution: Cytoskeleton and cytosol Function

GFAP is a class-III intermediate filament and a structural constituent of the cytoskeleton. It is a cell-specific marker that is used to distinguish astrocytes from other glial cells during the development of the CNS.

Cell Type
Astrocytes
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Gene ID
2670 View all products for this Gene ID
UniProt
View information about GFAP on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 7    Revision Date: 09/25/2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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