Purified anti-CD105 (Endoglin) Antibody

Pricing & Availability
Clone
O99E5 (See other available formats)
Regulatory Status
RUO
Other Names
Endoglin
Isotype
Mouse IgG1
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Product Citations
publications
99E5_PURE_CD105_Endoglin_Antibody_WB_080316
Total cell lysate from HUVEC cells (lane 1, 15 µg) and THP-1 cells (lane 2, 15 µg) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with purified anti-CD105 (Endoglin) antibody (clone O99E5). Proteins were visualized using an HRP Goat anti-mouse IgG Antibody and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control.
  • 99E5_PURE_CD105_Endoglin_Antibody_WB_080316
    Total cell lysate from HUVEC cells (lane 1, 15 µg) and THP-1 cells (lane 2, 15 µg) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with purified anti-CD105 (Endoglin) antibody (clone O99E5). Proteins were visualized using an HRP Goat anti-mouse IgG Antibody and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control.
Cat # Size Price Save
684102 100 µg ¥21,000
Description

CD105 (Endoglin) is expressed by endothelial cells, pro-fibrogenic cells and hepatic stellate cells; it plays important roles in endothelial proliferation and cardiovascular development. Mutations in the ENG gene lead to Hereditary Hemorrhagic Telangiectasia, a disorder characterized by arteriovenous malformations. An extracellular domain of the full-length membrane endoglin, soluble endoglin, is a proposed biomarker of cardiovascular disease progression, cardiovascular disease treatment and endothelial dysfunction. Endoglin is also expressed in the syncytiotrophoblast throughout pregnancy, but transiently upregulated in the extravillous trophoblast of anchoring villi. Furthermore, endoglin is overexpressed in the tumor-associated vascular endothelium, where it modulates angiogenesis.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length recombinant protein of human Endoglin/CD105 expressed in HEK293T.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Does not react with mouse.

RRID
AB_2572089 (BioLegend Cat. No. 684102)

Antigen Details

Structure
658 amino acid with the predicted molecular weight of approximately 71kD.
Distribution

Endothelial cells, mesangial cells, cardiac and scleroderma fibroblasts, and hepatic stellate cells.

Function
Endoglin plays important roles in endothelial proliferation and cardiovascular development.
Interaction
TGFBR1, TGFBR2, TGF-beta 1, TCTEX1D4, ARRB2 and GDF2.
Ligand/Receptor
TGF-β1, TGF-β3.
Cell Type
Endothelial cells, Fibroblasts
Biology Area
Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Immunology
Molecular Family
CD Molecules
Antigen References

1. Rathouska J, et al. 2015. Atherosclerosis 243:383.
2. Kapur NK, et al. 2013. Vasc. Health Risk Manag. 9:195.
3. Meurer SK, et al. 2014. World J. Biol. Chem. 5:180.
4. Gregory AL, et al. 2014. Placenta 35:S93-9.
5. Nassiri F, et al. 2011. Anticancer Res. 31:2283.
5. Zhang Y, et al. 2011. Int. J. Clin. Exp. Med. 4:32-42.

Gene ID
2022 View all products for this Gene ID
UniProt
View information about CD105 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 08/03/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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