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Purified anti-β-Amyloid, 1-16 Antibody (Previously Covance catalog# SIG-39322)

Pricing & Availability
Clone
Poly8029 (See other available formats)
Regulatory Status
RUO
Other Names
AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, Amyloid beta A4 protein, preA4, protease nexin-II, peptidase nexin-II, beta-amyloid peptide, alzheimer disease amyloid protein, cerebral vascular amyloid peptide, APP, Amyloid Precursor Protein
Previously
Signet Catalog# 9322-005
Covance Catalog# SIG-39322
Isotype
Rabbit Polyclonal IgG
Ave. Rating
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Product Citations
publications
Poly8029_Pure_B-Amyloid_Antibody_110817
IHC staining of anti-β-Amyloid, 1-16 antibody (clone Poly8029) on formalin-fixed paraffin-embedded Human Alzheimer's disease brain tissue. Following antigen retrieval using 70% formic acid, the tissue was incubated with 1 µg/mL of the primary antibody for 60 minutes at room temperature. BioLegend’s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm.
  • Poly8029_Pure_B-Amyloid_Antibody_110817
    IHC staining of anti-β-Amyloid, 1-16 antibody (clone Poly8029) on formalin-fixed paraffin-embedded Human Alzheimer's disease brain tissue. Following antigen retrieval using 70% formic acid, the tissue was incubated with 1 µg/mL of the primary antibody for 60 minutes at room temperature. BioLegend’s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm.
Cat # Size Price Save
802901 50 µg ¥111,320
Description

Amyloid beta (Aβ or Amyloid beta) denotes peptides of 36-43 amino acids in length that are crucially involved in Alzheimer's disease as the main component of the amyloid plaques found in the brains of Alzheimer patients. The peptides result from the amyloid precursor protein (APP), which is cut by certain enzymes to yield Aβ. Aβ molecules can aggregate to form oligomers (known as "seeds") which are believed to be able to induce other Aβ molecules to also take the misfolded oligomeric form, leading to a chain reaction akin to a prion infection. The seeds or the resulting amyloid plaques are toxic to nerve cells. The other protein implicated in Alzheimer's disease, tau protein, also forms such prion-like misfolded oligomers, and there is some evidence that misfolded Aβ can induce tau to misfold.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Polyclonal
Host Species
Rabbit
Formulation
Phosphate-buffered solution (no preservatives or carrier proteins).
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

IHC-P - Quality tested
ELISA, WB - Reported in the literature, not verified in house 
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1-5 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting, immunohistochemistry (IHC), and ELISA.

 

This antibody reacts with all isoforms of beta amyloid. It was developed using beta amyloid peptide (amino acids 1-16) conjugated to KLH.

 

This product may contain other non-IgG subtypes.

 

Pre-treatment: For optimal staining, tissue sections should be pre-treated with an antigen unmasking solution such as 70% Formic acid.

 

Positive control: Human Alzheimer’s disease brain tissue.

Application References

(PubMed link indicates BioLegend citation)
  1. Robertson RT, et al. 2009. Neuroscience 3:1334-46. (IHC) PubMed
  2. Chen KF, et al. 2014. Dis Model Mech. 4:445 (IHC) PubMed
Product Citations
  1. Younan ND, et al. 2018. J Biol Chem. 293:13090. PubMed
  2. Chen K, et al. 2014. Dis Model Mech. 7:445-458. PubMed
  3. Robertson R, et al. 2009. Neuroscience. 164:1334-1346. PubMed
  4. Condello C, et al. 2018. Proc Natl Acad Sci U S A. 115:E782. PubMed
RRID
AB_2564699 (BioLegend Cat. No. 802901)

Antigen Details

Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
APP/β-Amyloid
Gene ID
351 View all products for this Gene ID
UniProt
View information about beta-Amyloid 1-16 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 02/09/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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