Purified anti-β-Amyloid, 1-11 Antibody

Pricing & Availability
Clone
NAB 228 (See other available formats)
Other Names
Amyloid beta A4 protein, preA4, protease nexin-II, peptidase nexin-II, beta-amyloid peptide, alzheimer disease amyloid protein, cerebral vascular amyloid peptide, APP, Amyloid Precursor Protein
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
NAB228_PURE_betaAmyloid_Antibody_IHC-P_100616
IHC staining of anti-β-Amyloid, 1-11 antibody (clone NAB 228) on formalin-fixed paraffin-embedded human Alzheimer's disease neocortical brain tissue. After antigen retrieval using 70% Formic Acid for 20 minutes, the tissue was incubated with the primary antibody at 10 µg/ml for 24 hours at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit was used for detection followed by hematoxylin counterstaining.
  • NAB228_PURE_betaAmyloid_Antibody_IHC-P_100616
    IHC staining of anti-β-Amyloid, 1-11 antibody (clone NAB 228) on formalin-fixed paraffin-embedded human Alzheimer's disease neocortical brain tissue. After antigen retrieval using 70% Formic Acid for 20 minutes, the tissue was incubated with the primary antibody at 10 µg/ml for 24 hours at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit was used for detection followed by hematoxylin counterstaining.
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Cat # Size Price Save
835104 100 µg ¥56,400
Description

Alzheimer’s disease is characterized by the accumulation of aggregated Aβ peptides in senile plaques and vascular deposits. Aβ peptides are derived from amyloid precursor proteins (APPs) through sequential proteolytic cleavage of APP by β-secretases and γ-secretases generating diverse species of Aβ with differential propensities to fibrillize and deposit in Senile Plaques.

According to the UniProtKB database, APP (ID# P05067) has 11 isoforms (34 to ~90 kD). The 770 amino acid form (APP770) has been designated as the canonical form. Isoform APP695 is the predominant form expressed in neuronal tissue. Isoforms APP751 and APP770 are widely expressed in non-neuronal cells. Isoform APP751 is the most abundant form in T-lymphocytes.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was raised against a peptide sequence corresponding to amino acids 1-11 of human amyloid beta (Aβ) protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
ELISA Detection - Validated
IP, WB - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.5 - 5.0 µg/ml is suggested. For ELISA Detection, a concentration range of 25 - 50 ng/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the releavant formats) include immunoprecipication4, Western blotting1,2,4,9, and immunoflurescence microscopy7.

NAB 228 recognizes a peptide corresponding to amino acid residues 1-11 of the human amyloid beta (Aβ) as determined by ELISA using peptides that block its immunoreactivity. This antibody also recognizes full length amyloid precursor protein (APP), soluble APP form (sAPPα), C99 cleavage form, and Aβ (1-40 and 1-42). NAP 228 does not recognize sAPPβ.

This antibody can be used to stain amyloid plaques (diffuse and compact) and amyloid angiopathy on formalin-fixed paraffin-embedded tissue (human AD, Downs syndrome, MCI and transgenic mouse).

Application References

(PubMed link indicates BioLegend citation)
  1. Lee EB, et al. 2003. J. Biol. Chem. 278(7):4458-66. (WB) PubMed
  2. Mandler M, et al. 2015. PLoS One 1:e0115237. (WB) PubMed
  3. Wolk DA, et al. 2011. Arch. Neurol. 11:1398. (IHC) PubMed
  4. Lee EB, et al. 2006. J. Biol. Chem. 7:4292. (IP, WB, IHC) PubMed
  5. Berlau DJ, et al. 2013. Alzheimers Dement. 6:699. (IHC) PubMed
  6. Robinson JL, et al. 2014. Brain 137:2578. (IHC) PubMed
  7. Carroll JC, et al. 2011. J. Neurosci. 40:14436. (IHC, IF) PubMed
  8. Hurtado DE, et al. 2010. Am. J. Pathol. 4:1977. (IHC) PubMed
  9. Lee EB, et al. 2005. J. Cell Biol. 2:291. (IHC, WB) PubMed
RRID
AB_2632642 (BioLegend Cat. No. 835104)

Antigen Details

Structure
Amyloid precursor protein is a 770 amino acid protein with a molecular mass of approximately 90 kD. Aß denotes peptides of 36-43 amino acids generated from cleavage of APP by secretases. Aß has an apparent molecular mass of about 4 kD.
Distribution

Cellular distribution: cytosol, extracellular, endosomes, golgi apparatus.

Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
APP/β-Amyloid
Gene ID
351 View all products for this Gene ID
UniProt
View information about beta-Amyloid, 1-11 on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 05/01/2017

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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