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Purified anti-ATF1 Antibody

Pricing & Availability
Clone
W16033B (See other available formats)
Regulatory Status
RUO
Other Names
ATF1, TREB36
Isotype
Rat IgG2a, λ
Ave. Rating
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Product Citations
publications
W16033B_Purified_ATF1_Antibody_041919.png
Western blot analysis of 15µg cell lysates from human HeLa, U87-MG, Daudi, A549, Raw264.7 (Mouse) and UMR106 (Rat) cells. Samples were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, probed with 1 µg/mL anti-ATF1 antibody, clone W16033B, followed by incubation with horseradish peroxidase (HRP) labeled goat anti-rat secondary antibody (Cat. No. 405405, 1:3000 dilution). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904, 1:10000 dilution) was used as a loading control (Cat. No. 664804, 1:25000 dilution). Ponceau S staining was used as a loading control. Western-Ready™ ECL Substrate Kit (Cat. No. 426303) was used for developing.
  • W16033B_Purified_ATF1_Antibody_041919.png
    Western blot analysis of 15µg cell lysates from human HeLa, U87-MG, Daudi, A549, Raw264.7 (Mouse) and UMR106 (Rat) cells. Samples were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, probed with 1 µg/mL anti-ATF1 antibody, clone W16033B, followed by incubation with horseradish peroxidase (HRP) labeled goat anti-rat secondary antibody (Cat. No. 405405, 1:3000 dilution). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904, 1:10000 dilution) was used as a loading control (Cat. No. 664804, 1:25000 dilution). Ponceau S staining was used as a loading control. Western-Ready™ ECL Substrate Kit (Cat. No. 426303) was used for developing.
Cat # Size Price Save
666501 25 µg ¥22,220
666502 100 µg ¥55,660
Description

ATF1 is a transcription factor regulated by multiple signaling pathways. For example, ERK1/2 and p38 MAPK could phosphorylate and activate ATF1. Activated ATF1  could then regulate downstream targets to mediate cell growth and proliferation. Chimeric proteins of ATF1 fused with FUS or EWSR1 have been discovered in angiomatoid fibrous histiocytoma and clear cell sarcoma.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human recombinant Full length ATF1 Protein expressed in E. Coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 2 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2810696 (BioLegend Cat. No. 666501)
AB_2810697 (BioLegend Cat. No. 666502)

Antigen Details

Distribution

Nucleus

Function
Transcription factor
Biology Area
Cell Biology, Cell Proliferation and Viability, Signal Transduction, Transcription Factors
Molecular Family
Nuclear Markers
Gene ID
466 View all products for this Gene ID
UniProt
View information about ATF1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 04/19/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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