PerCP/Cyanine5.5 anti-mouse CD278 (ICOS) Antibody

Pricing & Availability
7E.17G9 (See other available formats)
Regulatory Status
Other Names
Inducible COStimulatory molecule, H4
Rat IgG2b, κ
Ave. Rating
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Product Citations
ConA-stimulated (3 days) C57BL/6 splenocytes were stained with CD3 APC and CD278 (ICOS) (clone 7E.17G9) PerCP/Cyanine5.5 (left) or rat IgG2b, ? PerCP/Cyanine5.5 isotype control (right).
  • 7E-17G9_PerCPCyanine55_CD278_Antibody_012120
    ConA-stimulated (3 days) C57BL/6 splenocytes were stained with CD3 APC and CD278 (ICOS) (clone 7E.17G9) PerCP/Cyanine5.5 (left) or rat IgG2b, ? PerCP/Cyanine5.5 isotype control (right).
Compare all formats See PerCP/Cyanine5.5 spectral data
Cat # Size Price Save
117423 25 µg ¥25,960
117424 100 µg ¥71,060

The 7E.17G9 antibody reacts with the 47-57 kD ICOS protein, also known as inducible costimulatory molecule, and H4. This protein is homologous to the CD28/CTLA-4 proteins. ICOS is expressed on activated T cells and a subset of thymocytes and can costimulate T cells and induce proliferation. In addition ICOS has been shown to be involved in humoral immune responses (B cell germinal center formation). The ICOS ligand, B7h/B7RP-1 and B7-H2 is constitutively expressed in B cell areas of secondary lymphoid organs and can be induced in other tissues by LPS. ICOS stimulation has been shown to potentiate TCR-mediated IL-4 and IL-10 production and has been proposed to play a role in Th2 cell development. ICOS stimulation has been shown to be involved in airway tolerance and the downregulation of pulmonary inflammation.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Antibody Type
Host Species
Mouse ICOS cDNA and ICOS hexahistidine fusion protein
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm. 

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: blocking of ligand binding. The LEAF™ format is suggested for blocking studies.

Application References

(PubMed link indicates BioLegend citation)
  1. Akbari O, et al. 2002. Nat. Med. 8:1024.
  2. Harada H, et al. 2003. J. Clin. Invest. 112:234.
  3. McAdam AJ, et al. 2000. J. Immunol. 165:5035. (FC Block)
  4. Tan SL, et al. 2006. J. Immunol. 176:2872. PubMed
AB_2832418 (BioLegend Cat. No. 117423)
AB_2832419 (BioLegend Cat. No. 117424)

Antigen Details

CD28/CTLA-4, 47-57kD

Activated T cells, subset of thymocytes

Costimulates T cell activation, proliferation, humoral immune response
Cell Type
T cells, Thymocytes
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Rudd CE, et al. 2003. Nat. Rev. Immunol. 3:544.
2. McAdam AJ, et al. 2000. J. Immunol. 165:5035.
3. Mak TW, et al. 2003. Nat. Immunol. 4:765

Gene ID
54167 View all products for this Gene ID
View information about CD278 on

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 01/21/2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Biotin anti-mouse CD278 (ICOS)

  • PE anti-mouse CD278 (ICOS)

  • TotalSeq™-A0847 anti-mouse CD278 (ICOS)

  • TotalSeq™-C0847 anti-mouse CD278 (ICOS)

  • Ultra-LEAF™ Purified anti-mouse CD278 (ICOS)

  • PE/Cyanine7 anti-mouse CD278 (ICOS)

  • PerCP/Cyanine5.5 anti-mouse CD278 (ICOS)

  • APC anti-mouse CD278 (ICOS)

  • TotalSeq™-B0847 anti-mouse CD278 (ICOS)

  • Spark Red™ 718 anti-mouse CD278 (ICOS)


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