PerCP/Cyanine5.5 Streptavidin

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Regulatory Status
RUO
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Product Citations
publications
Steptavadin_PerCpCyanine55_101818
Human peripheral blood lymphocytes were stained with biotinylated CD3, followed by SAV-PerCP/Cyanine5.5 (filled histogram), or directly stained with SAV-PerCP/Cyanine5.5 (open histogram).
  • Steptavadin_PerCpCyanine55_101818
    Human peripheral blood lymphocytes were stained with biotinylated CD3, followed by SAV-PerCP/Cyanine5.5 (filled histogram), or directly stained with SAV-PerCP/Cyanine5.5 (open histogram).
See PerCP/Cyanine5.5 spectral data
Cat # Size Price Save
405214 100 µg ¥57,750
Description

Streptavidin binds to biotin with high affinity. Streptavidin-PerCP/Cyanine5.5 is useful for detecting biotinylated antibodies. The excitation of PerCP/Cyanine5.5 by 488 nm laser light induces a light emission maximum of 690 nm.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat, All Species
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
Streptavidin is conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The Streptavidin-PerCP/Cyanine5.5 solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

ICFC - Verified

Recommended Usage

Each lot of this Streptavidin-PerCP/Cyanine5.5 is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the PerCP/Cyanine5.5 conjugation. For immunofluorescent staining, the recommended use of this reagent is = 0.06 µg per 106 cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Streptavidin-PerCP/Cyanine5.5 is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-PerCP/Cyanine5.5 is 110 kD and Streptavidin alone is 52 kD.

Additional Product Notes

BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.

Application References

(PubMed link indicates BioLegend citation)
  1. Killeen ME, et al. 2013. J. Immunol. 190:4324. PubMed
  2. Grage-Griebenow E, et al. 2014. Mol Oncol. 8:982. PubMed
  3. Mise-Omata S, et al. 2014. Int Immunol. 26:607. PubMed
Product Citations
  1. Suzuki M, et al. 2015. J Immunol. 195: 1273-1281. PubMed
  2. Zakeri A, et al. 2021. J Extracell Vesicles. 10:e12131. PubMed
  3. Grage-Griebenow E, et al. 2014. Mol Oncol. 8:982. PubMed
  4. Hirano KI, et al. 2021. eLife. 0.416666666666667. PubMed
  5. Pease NA, et al. 2021. Cell Reports. 34(12):108888. PubMed
  6. Greenblatt SM, et al. 2018. Cancer Cell. 33:1111. PubMed
  7. Ng KK et al. 2018. eLife. 7 pii: e37851. PubMed
  8. Perelman SS, et al. 2021. Nat Microbiol. 6:731. PubMed
  9. Dong S, et al. 2021. Nature. 591:117. PubMed
  10. Glaros V, et al. 2021. Immunity. 54:2005. PubMed
  11. Wittner J, et al. 2022. Mucosal Immunol. 15:668. PubMed
  12. Fournier M, Bijl C 2015. Stem Cells Dev. 24: 2413-2422. PubMed
  13. Delacher M, et al. 2020. Immunity. 52:295. PubMed
  14. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  15. Fujii T, et al. 2021. Bone Res. 9:4. PubMed
  16. Verheijen M, et al. 2020. Cell Rep. 108376:33. PubMed
  17. Yuan X, et al. 2017. Elife. 6:e29540. PubMed
  18. Pi J, et al. 2022. J Nanobiotechnology. 20:36. PubMed
  19. Mise-Omata S, et al. 2014. Int Immunol. 26:607. PubMed
  20. Lopes N, et al. 2022. Elife. 11:. PubMed
  21. Wagner AK, et al. 2022. iScience. 25:105137. PubMed
  22. Yoshinaga M, et al. 2022. Nat Commun. 13:6435. PubMed
  23. Killeen M, et al. 2013. J Immunol. 190:4324. PubMed
  24. Li X, et al. 2019. Stem Cells. 37:937. PubMed
  25. Furusawa J, et al. 2016. PLoS Pathog. 12: 1005507. PubMed
  26. Boguslawski KM, et al. 2020. Sci Adv. 6:eaax7515. PubMed
RRID
AB_2716577 (BioLegend Cat. No. 405214)

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 5    Revision Date: 06/18/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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