PerCP/Cyanine5.5 anti-mouse CD45.2 Antibody

Pricing & Availability
Clone
104 (See other available formats)
Regulatory Status
RUO
Other Names
Ly-5.2, LCA
Isotype
Mouse (SJL) IgG2a, κ
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Product Citations
publications
104_PerCPCy5dot5_011108
C57BL/6 mouse splenocytes stained with 104 PerCP/Cyanine5.5
  • 104_PerCPCy5dot5_011108
    C57BL/6 mouse splenocytes stained with 104 PerCP/Cyanine5.5
Compare all formats See PerCP/Cyanine5.5 spectral data
Cat # Size Price Save
109827 25 µg ¥16,170
109828 100 µg ¥52,080
Description

CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
B10.S mouse thymocytes and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation4, in vivo and in vitro blocking of B cell responses1,2, and immunohistochemical staining of acetone-fixed frozen sections3

Additional Product Notes

BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.

Application References

(PubMed link indicates BioLegend citation)
  1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
  2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
  3. Suzuki K, et al. 2000. Immunity 13:691. (IHC)
  4. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
  5. Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837.
  6. Pascal V, et al. 2007. J. Immunol. 179:1751.
  7. Burman AC, et al. 2007. Blood 110:1064.
  8. Kincaid EZ, et al. 2007. J. Immunol. 179:3187.
  9. Phan TG, et al. 2007. Nature Immunol. 8:992.
  10. Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed
  11. Wen T, et al. 2013. PNAS. 110:6067. PubMed
  12. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations
  1. Wang Y, et al. 2021. Cancer Res. 81:174. PubMed
  2. Charpentier JC, et al. 2020. Nat Commun. 11:180. PubMed
  3. Dong MB, et al. 2020. Cell. 178(5):1189-1204.e23.. PubMed
  4. Harsha Krovi S, et al. 2020. Nat Commun. 4.790277778. PubMed
  5. Hirai T, et al. 2020. Immunity. 54(1):84-98.e5. PubMed
  6. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
  7. Zhang LJ, et al. 2019. Immunity. 50:121. PubMed
  8. Emal D, et al. 2016. J Am Soc Nephrol. 10.1681/ASN.2016030255. PubMed
  9. Scalzo S, et al. 2022. Int J Nanomedicine. 17:2865. PubMed
  10. Turner JS et al. 2018. Cell reports. 25(6):1395-1403 . PubMed
  11. Stewart I et al. 2018. Immunity. 49(3):477-489 . PubMed
  12. Fu B et al. 2017. Immunity. 47(6):1100-1113 . PubMed
  13. Xia S, et al. 2014. J Leukoc Biol. 95:733. PubMed
  14. Yu K, et al. 2021. Cell Reports. 35(12):109273. PubMed
  15. Isoda T et al. 2017. Cell. 171(1):103-119 . PubMed
  16. Yuzhu Hou et al. 2018. Immunity. 49(3):490-503 . PubMed
  17. Low MSY, et al. 2019. Cell Reports. 29(9):2634-2645.e5. PubMed
  18. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  19. Rothchild AC, et al. 2020. Bio Protoc. 10:00. PubMed
  20. Kleinholz CL, et al. 2021. Sci Rep. 11:15071. PubMed
  21. Yang F, et al. 2020. Cancer Res. 80:3677. PubMed
  22. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  23. Rodriguez-Ruiz M, et al. 2016. Cancer Res . 76: 5994 - 6005. PubMed
  24. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  25. Stivala S, et al. 2019. J Clin Invest. 130:1596. PubMed
  26. Samarchith P Kurup et al. 2019. Cell host & microbe. 25(4):565-577 . PubMed
  27. Ramos CV, et al. 2020. Cell Reports. 32(3):107910. PubMed
  28. Yap JY, et al. 2018. Immunology. 154:522. PubMed
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  30. Chen Z et al. 2019. Immunity. 51(5):840-855 . PubMed
  31. Hulsmans M et al. 2017. Cell. 169(3):510-522 . PubMed
  32. Schadt L, et al. 2020. Cell Reports. 29(5):1236-1248.e7.. PubMed
  33. Zhang K, et al. 2014. PLoS Pathog. 10:1004385. PubMed
  34. Venturutti L, et al. 2020. Cell. 182(2):297-316.e27. PubMed
  35. Stotesbury C, et al. 2020. J Immunol. 204:1582. PubMed
  36. Xu S, et al. 2021. Immunity. . PubMed
  37. Kan X, et al. 2015. Cardiovasc Res. 10.1093/cvr/cvv255. PubMed
  38. Fraccarollo D, et al. 2021. eLife. 0.416666666666667. PubMed
  39. Bates PD, et al. 2021. Front Immunol. 12:668307. PubMed
  40. Liu C et al. 2019. Immunity. 51(2):381-397 . PubMed
  41. Olson GS, et al. 2021. Cell Reports. 35(9):109195. PubMed
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  43. Evgin L, et al. 2022. Sci Transl Med. 14:eabn2231. PubMed
  44. Evgin L, et al. 2020. Nat Commun. 2.671527778. PubMed
  45. Melo-Silva CR, et al. 2021. PLOS Pathogens. 17(5):e1009593. PubMed
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  50. Wang X, et al. 2016. J Immunol. 197(12):4750-4761. PubMed
  51. Forero A, et al. 2020. Immunity. 51(3):451-464. PubMed
  52. Chen Z, et al. 2021. Cell. 184(5):1262-1280.e22. PubMed
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  54. Xu Y, et al. 2021. iScience. 24:103445. PubMed
  55. Hoyer FF, et al. 2020. Immunity. 51(5):899-914.e7.. PubMed
  56. Gundra UM, et al. 2017. Nat Immunol. 1.195833333. PubMed
  57. Cao H, et al. 2020. Front Pharmacol. 11:578943. PubMed
  58. Martins R, et al. 2016. Nat Immunol. 17:1361-1372. PubMed
  59. Ikeda T, et al. 2014. PLoS One. 9:115198. PubMed
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RRID
AB_893352 (BioLegend Cat. No. 109827)
AB_893350 (BioLegend Cat. No. 109828)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Suzuki K, et al. 2000. Immunity 13:691.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45.2 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 2    Revision Date: 09/18/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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